We narrowed to 6,356 results for: sas

1. pMT1-24X-NOG(AtUBI10)

   Plasmid

   #202016

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Inserts

   dCas9-24XGP41
   NbGP41P-sfGFP-VP64-GB1
   

   Use

   Tags

   GB1 and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

   Plasmid

   #236612

   Purpose

   Knockouts ANKRD11 in human cells

   Depositor

   Tadashi Nakagawa

   Article

   Sashiyama et al iScience 2025 May 19, 112699

   Insert

   ANKRD11 (ANKRD11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

   Plasmid

   #236608

   Purpose

   Knockouts Ankrd11 in mouse cells

   Depositor

   Tadashi Nakagawa

   Article

   Sashiyama et al iScience 2025 May 19, 112699

   Insert

   Ankrd11 (Ankrd11 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TA-ERN-WDR5-FLAG

   Plasmid

   #236721

   Purpose

   Expresses human WDR5 in mammalian cells

   Depositor

   Tadashi Nakagawa

   Article

   Sashiyama et al iScience 2025 May 19, 112699

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Piggybac transposon

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

   Plasmid

   #209298

   Purpose

   A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.

   Depositor

   Birgitt Schuele

   Article

   Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1

   Inserts

   dCas9
   tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-inducible promoter (TRE3G)

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. phBG-P53DD

   Plasmid

   #149707

   Purpose

   In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

   Depositor

   Erika Sasaki

   Article

   Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Rna transcription

   Tags

   Expression

   Mutation

   deleted amino acid 14-301

   Promoter

   T7

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234370

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

   Plasmid

   #234371

   Purpose

   T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis Ubi10 and MTAP1 (synthetic)

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234372

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-24XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII

    Plasmid

    #192705

    Purpose

    Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728

    Depositor

    Sascha Martens

    Inserts

    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16L1
    

    Use

    Tags

    -GFP-TEV-StrepII and 10x-His-TEV

    Expression

    Insect

    Mutation

    F32A-I35A-I36A

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSH1/M-FGFR3-Fv-Fvls-E

    Plasmid

    #15287

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
    

    Use

    Tags

    Myristoylation-targeting domain c-Src (14aa) and …

    Expression

    Mammalian

    Mutation

    FGFR3 cytoplasmic domain only FKBP12 F36V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC-sleeping beauty

    Plasmid

    #65487

    Purpose

    HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

    Depositor

    Guillaume Filion

    Insert

    sleeping beauty transposase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    HSP

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKS1024

    Plasmid

    #239495

    Purpose

    Express peroxisomal RsAS

    Depositor

    John Dueber

    Insert

    Ubi-Y-RsAS-Venus-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. psfTn5

    Plasmid

    #79107

    Purpose

    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

    Depositor

    Sten Linnarsson

    Insert

    transposase 5
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E54K and L372P

    Promoter

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKS1025

    Plasmid

    #239496

    Purpose

    Express inactive peroxisomal RsAS as negative control

    Depositor

    John Dueber

    Insert

    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H360E

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJY4092

    Plasmid

    #113334

    Purpose

    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Insert

    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIP4_3ILS

    Plasmid

    #234215

    Purpose

    Heterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3ILS
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGAD424-hL1

    Plasmid

    #89423

    Purpose

    Two-hybrid system

    Depositor

    Fritz Rathjen

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Gal4 AD

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKC-Tol2

    Plasmid

    #85600

    Purpose

    Source of Tol2 transposase driven by a mini-CAGS promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mini-CAG

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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