We narrowed to 7,533 results for: ski

1. pcDNA5-FRT-TO-Nsp16-HF

   Plasmid

   #157725

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16-HF (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA5-FRT-TO-Nsp16

   Plasmid

   #157723

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16 (ORF1ab SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGEX6P1-HA-MEK1

   Plasmid

   #53159

   Purpose

   Expresses GST-HA tagged human MEK1 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Tags

   GST and HA

   Expression

   Bacterial

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY1462_SERPINA1_CCA32_Caspase

   Plasmid

   #193194

   Purpose

   Express Caspase conditional upon RNA transcript

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   iCaspase9 (CASP9 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBeta-lacZ-attBx2

   Plasmid

   #124445

   Purpose

   Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Type

   Empty backbone

   Use

   Enhancer reporter assay construct

   Tags

   LacZ

   Expression

   Mammalian

   Promoter

   beta globin minimal promoter

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-CMV-FLAG-CFP-AMOT

   Plasmid

   #235682

   Purpose

   Express CFP tagged AMOT gene in mammalian cells using the CMV promoter

   Depositor

   Duojia Pan

   Article

   Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

   Insert

   AMOT (AMOT Human)
   

   Use

   Lentiviral

   Tags

   FLAG-CFP

   Expression

   Mammalian

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEND-184_Cacnes_mCherry

   Plasmid

   #225610

   Purpose

   Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEND-164_Cacnes_pFAST

   Plasmid

   #225616

   Purpose

   Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   pFAST
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-R-iLACCO1.1

    Plasmid

    #208100

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.2
    

    Use

    AAV

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-CMV-V5-RFP-SLMAP (isoform r)

    Plasmid

    #235685

    Purpose

    Express RFP tagged SLMAP gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    SLMAP (SLMAP Human)
    

    Use

    Lentiviral

    Tags

    V5-RFP

    Expression

    Mammalian

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

    Plasmid

    #194694

    Purpose

    CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLYS6-METTL17-CtoS-FLAG

    Plasmid

    #220876

    Purpose

    Expressing METTL17-FLAG_Cys to Ser mutants

    Depositor

    Vamsi Mootha

    Article

    Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

    Insert

    METTL17 (METTL17 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Cys to Ser

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX SHP-1(NC)-SH2

    Plasmid

    #46496

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(NC) (PTPN6 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-221

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. HH02-LP Clone 2 heavy chain

    Plasmid

    #192175

    Purpose

    Clone 2 heavy chain (HH02)

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 heavy chain (HH02)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. HK02-LP Clone 2 light chain

    Plasmid

    #192179

    Purpose

    Clone 2 light chain (HK02)

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 light chain (HK02)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEND-224_Cacnes_sfGFP

    Plasmid

    #225611

    Purpose

    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. HiBiT-FZD8

    Plasmid

    #195846

    Purpose

    Expresses human FZD8 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD8 (FZD8 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hSyn1_nLightR

    Plasmid

    #187180

    Purpose

    Expresses red norepinephrine indicator nLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Has Service

    AAV9

    Insert

    nLightR
    

    Use

    AAV

    Tags

    Flag tag

    Promoter

    human Synapsin-1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SFFV-DTAG-NTERM-GFP

    Plasmid

    #185760

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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