We narrowed to 7,237 results for: ALP

1. Go1-CASE

   Plasmid

   #168123

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at L91/G92 within GNAO1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p300 core-dCas9-CBP core

   Plasmid

   #179559

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

   Plasmid

   #172907

   Purpose

   AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Has Service

   AAV1

   Insert

   Voltron2-ST
   

   Use

   AAV and Cre/Lox

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   hSynapsin1

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Oliver Weichenrieder, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / Lac

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-mEGFP-RUNX1-WT

   Plasmid

   #194574

   Purpose

   Mammalian Expression of mEGFP-RUNX1 WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   RUNX1 (RUNX1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQ-Elo-HB-C-A(1-630)

   Plasmid

   #171086

   Purpose

   Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   Elongin B (ELOB Human)
   Elongin C (ELOC Human)
   Elongin A (ELOA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deleted N-term 26aa and C-term 142aa for mutant e…

   Promoter

   T5 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-PAK1

   Plasmid

   #23543

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PAK1 (PAK1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

   Plasmid

   #154089

   Purpose

   Improved version to express RUNX1, P2A, ERG in mammalian cells.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GCN5 core-dCas9-p300 core

   Plasmid

   #179553

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI

    Plasmid

    #198466

    Purpose

    Expresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-XBB.1.5 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-TRI-2A

    Plasmid

    #74674

    Purpose

    mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

    Depositor

    Roberto Giovannoni

    Article

    Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

    Inserts

    HO1 (HMOX1 Human)
    CD73 (NT5E Human)
    CD39 (ENTPD1 Human)
    

    Use

    Tags

    F2A

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQ-Elo-HB-C-A(400-772)

    Plasmid

    #171087

    Purpose

    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Inserts

    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Deleted N-term 26+399 amino acids for mutant expr…

    Promoter

    T5 promoter and T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQ-Elo-HB-C-A(1-772)

    Plasmid

    #171085

    Purpose

    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Inserts

    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Deleted N-term 26 amino acids for expression purp…

    Promoter

    T5 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-AKT1

    Plasmid

    #23752

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pDONR223-ZAK

    Plasmid

    #23709

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ZAK (MAP3K20 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI

    Plasmid

    #181695

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.1.529 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GCN5 FL-dCas9-CBP core

    Plasmid

    #179556

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GCN5 core-dCas9-CBP core

    Plasmid

    #179555

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRC8400-SARS-CoV-2-S2P-XBB-AVI

    Plasmid

    #196622

    Purpose

    Expresses SARS-CoV-2 S2P protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-XBB (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAdeasy-CMV-AE-EF1α-R2

    Plasmid

    #194928

    Purpose

    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis

    Depositor

    Limei Wang

    Article

    Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.

    Inserts

    AE
    EEF1A1 (EEF1A1 Human)
    R2
    

    Use

    Adenoviral

    Tags

    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c

    Expression

    Mutation

    a 48 bp linker (Gly3ser)4 was added between the t…

    Promoter

    CMV and EF1a

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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