We narrowed to 11,999 results for: SHA

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hiSIIN

Plasmid

#185662

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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midSIIN

Plasmid

#185665

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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dimSIIN

Plasmid

#185661

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHHM.X513-iNicSnFR3b-(V7)

Plasmid

#124881

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3b-(V7)

Tags

HA Tag, His Tag, and NA

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3- myc NES-mCE(K294A) NLS

Plasmid

#82467

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NLS deleted from mouse Capping Enzyme (Rngtt Mouse)

Expression

Mammalian

Mutation

deletion of KRKY from 573-657

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)

Plasmid

#70232

Purpose

ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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TMEM16A-I546K-peGFP-N1

Plasmid

#228582

Purpose

Expresses mouse TMEM16A-I546K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

I546K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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p23-NES-LwaCas13a-msfGFP-NES-Flag

Plasmid

#165070

Purpose

overexpression of LwaCas13a in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

LwaCas13a

Use

Lentiviral

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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loSIIN

Plasmid

#185663

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 WT

Plasmid

#69811

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_mKate2

Plasmid

#96942

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

far-red fluorescent protein

Use

Lentiviral

Mutation

E87D (please see depositor comments below)

Promoter

CaMKIIa

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-MAPTau-N-10

Plasmid

#55312

Purpose

Localization: Microtubules, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MAPTau (MAPT Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TIA1 RRM-mCherry-iLIDx6

Plasmid

#103782

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-mCherry-iLIDx6

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4(glu)

Plasmid

#14813

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(glu) (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Glu Thr-224Glu

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pLXSN-Axl

Plasmid

#65222

Purpose

Expression of Axl in mammalian cells

Depositor

Axel Ullrich

Article

Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

Insert

AXL (AXL Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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noSIIN

Plasmid

#185666

Purpose

shApc-expressing lentiviruses with no expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F2

Plasmid

#55785

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
CRISPseq-mCherry-backbone

Plasmid

#85708

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 DPP9S

Plasmid

#166789

Purpose

pDONR gateway shuttling vector with DPP9S (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Gateway shuttling vector

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1

Plasmid

#39281

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pCS2-hDKK2-flag

Plasmid

#15495

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK2 (DKK2 Human)

Tags

flag

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1

Plasmid

#39282

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pFE-Selo-FI-prk

Plasmid

#162698

Purpose

Expresses S. elongatus Form IB rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

S. elongatus Form IB rubisco and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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shTBX5 # 2

Plasmid

#42550

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
CamKIIa:smFP-TVA-RGV

Plasmid

#202594

Purpose

Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

Depositor

Oleksandr Shcheglovitov

Article

Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

Inserts

smFP
TVA
RGV

Use

Lentiviral

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-HA-TagBFP

Plasmid

#120910

Purpose

Expresses BFP tagged Ptch1-B

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA and TagBFP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221 DPP9L

Plasmid

#166790

Purpose

pDONR gateway shuttling vector with DPP9L (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Gateway shuttling vector

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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shTBX5 # 1

Plasmid

#42549

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-OsTIR1(WT)

Plasmid

#140729

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)-P2A-mAID-EGFP-NES

Use

AAV

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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