We narrowed to 10,115 results for: HA
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Plasmid#64876Purposelentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationS1977P, mimicking the chaos1 mutationPromoterEF1alphaAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-KATNA1
Plasmid#180650PurposeLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.DepositorInsertKATNA1 (KATNA1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GGACAGCACUCCCUUGAAAPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorInsertTET3-CxxCmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV E2F1 1-284
Plasmid#24216PurposeMammalian expression of human E2F1 (aa 1-284) with HA tagDepositorInsertE2F1 1-284 (E2F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196A
Plasmid#52974Purposeinsect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196A LKB1 phosphorylation defective mutantPromoterHsp70Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSExpressionMutationPromoterEF1a and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVE-FH-IN
Plasmid#70071Purpose2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cellsDepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
LAP2-beta-actin in modified pEGFP
Plasmid#34839DepositorInsertLAP2-beta-Actin (ACTB Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
Myopathy Loop Mutant Nuclear Myosin I YFP
Plasmid#60243PurposeExpresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activityDepositorInsertNuclear Myosin 1 (Myo1c Mouse)
UseTagsEYFP and NLSExpressionMammalianMutationDeleted I339, I340, G343, E344, E345,L346PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 AT1AR-TSTS/4A
Plasmid#45634DepositorInsertAT1AR-TSTS/A (Agtr1a Rat)
UseTagsHAExpressionMammalianMutationThr332, Ser335, Thr336, and Ser338 all changed to…PromoterCMVAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Myc-TRFcT
Plasmid#44573DepositorUseRetroviralTagsMycExpressionMammalianMutationTRF2 where TRFH domain (aa42-241) has been replac…PromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZJQIBEBT003
Plasmid#140053PurposeThe pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.DepositorInserteMutS-L157C-G233C-CBM-EGFP
UseTagsHis-TagExpressionBacterialMutationL157C, G233CPromoterT7Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP
Plasmid#154185Purposeexpresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cellsDepositorInsertretroCHMP3
UseTagsHA and T2A-H2B-BFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_ TET1 CXXC only
Plasmid#124084PurposeExpression of CXXC domain only form of TET1DepositorInsertTET1 - CXXC domain (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF1 alphaAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-hTERT-FF2
Plasmid#164910PurposeExpresses hTERT cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsHA tagExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXIP TGBRI K to R
Plasmid#19150DepositorInsertTransforming growth factor, beta receptor I (TGFBR2 Human)
UseRetroviralTagsHAExpressionMammalianMutationK420R mutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PiuA
Plasmid#128957Purposeexpresses Acinetobacter baumannii PiuA in E. coliDepositorInsertTonB-dependent siderophore receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756K
Plasmid#60727PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N1011Δ382-450
Plasmid#92322PurposeN-term of HCF-1 protein without amino acids 382-450DepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-1011 with internal deletion Δ382-450PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-C600
Plasmid#92324PurposeC-term of HCF-1 proteinDepositorInsertHCF-1 C-term 1436-2035 (HCFC1 Human)
UseTagsC-myc (EQKLISEED) and HAExpressionMammalianMutationAA 1436-2035PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-TAP_IRES_GFP
Plasmid#52740PurposeRetroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFPDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTags3x HA, 3x TEV, and BirA target siteExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNAmyc-hPR-B-486-933
Plasmid#89118Purposemammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domainsDepositorInserthuman progesterone receptor ligand binding domain (PGR Human)
UseTagsmycExpressionMammalianMutationcontains aa 486-933PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3F-CTD
Plasmid#105052PurposeOverexpression of APOBEC3F C-terminal domain in mammalian cell linesDepositorInsertAPOBEC3F (A3F) C-terminal domain (APOBEC3F Human)
UseTagsHA tagExpressionMammalianMutationit contains only C terminal domain of A3FPromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASt-SIK3 S563A
Plasmid#52976Purposeinsect expression of SIK3 S563A PKA phosphorylation defective mutantDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationS563A PKA phosphorylation defectivePromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756K
Plasmid#60731PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N748D
Plasmid#60726PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_3x_siRNA
Plasmid#59893Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has three siRNA-like targets (fully c…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
hFLT1 delta 754-763
Plasmid#86035Purposehuman VEGFR1 with a 10 amino acid deletion in EC close to TM domainDepositorInserthuman Flt1 delta754-763 aa (FLT1 Human)
UseTagsFLAG, HA, and MycExpressionMammalianMutationdelta 754-763PromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
[#GS16-LV] Syn1-MitoTRACER - Donor
Plasmid#233565PurposeLentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotorDepositorInsertMitoTRACER-Donor
UseLentiviral and Synthetic BiologyTagsGFP11 and HA TagExpressionMammalianMutationPromoterAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
MFE23-ZZ
Plasmid#223553PurposeMFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.DepositorInsertpET302-MFE23-ZZ
UseTagsHA tag and HIS tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(25Q)-c_myc
Plasmid#225228PurposeYeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.DepositorInsertsAga2
Htt (25Q)
UseTagsHA Tag and c-myc TagExpressionYeastMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(46Q)-c_myc
Plasmid#225229PurposeYeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.DepositorInsertsAga2
Htt (46Q)
UseTagsHA Tag and c-myc TagExpressionYeastMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-F-box:gs10:FRB:HA
Plasmid#228531PurposeF-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertF-box:gs10:FRB:HA (SKP2 Synthetic, Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA
Plasmid#228532PurposeA fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertmyc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-99a-WT
Plasmid#215880PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-SUN1ΔN
Plasmid#213508PurposeExpresses human eGFP-SUNΔN in mammalian cellsDepositorInserteGFP-SUNΔN
UseTagsExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only