We narrowed to 10,893 results for: ENA;
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Plasmid#91066PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE CDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-STChRger2-TS-EYFP
Plasmid#129393PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianMutationPromoterCaMKIIaAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
SauCas9 PE2*
Plasmid#169851PurposeMammalian Expression, sauCas9 based prime editorDepositorInsertSaCas9-N580A-M-MLV
UseTagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianMutationPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPseq-mCherry-backbone
Plasmid#85708PurposeBackbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1333 DddAtox C
Plasmid#158095Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1333 DddAtox N
Plasmid#158093Purposeexpresses the left-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-N–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU+UIM, aa 287-481)
Plasmid#61406PurposeExpresses human OTUD1 (OTU+UIM domains) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2103b
Plasmid#91062PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FlaviA-GFP-puro
Plasmid#140088PurposeFluorescence activatable reporter of flavivirus NS2B-NS3 protease activityDepositorInsertFlaviA-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianMutationPromoterCMVAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-LAP tag
Plasmid#67618PurposeA pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…TagsC-termnal LAP (eGFP-TEV-S)ExpressionMammalianMutationPromoterCMV and SP6Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mEGFP-NLS
Plasmid#205001PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM
Plasmid#183150PurposeExpression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)DepositorInsertsLex-ER-B112
Zif268 DBD - hPRLBD - MSN2AD
UseFor yeast genomic integrationTagsExpressionMutationPromoterAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Lck-FRB-mTurquoise2
Plasmid#176130PurposePlasma membrane localized FRB, rapamycin heterodimerizationDepositorInsertFRB
UseLentiviralTagsExpressionMutationPromoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHMT.wt (p53)
Plasmid#12139DepositorAvailable SinceJune 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
Plasmid#194222PurposeMammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptorsDepositorInsertSynNotch-GAL4 DBD-VP64 AD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-V0D1
Plasmid#210348PurposeExpresses ATP6V0D1 in mammalian cellsDepositorAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialMutationPromoterP(rhaB)Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWH-largeT
Plasmid#133895PurposePositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pBDepositorInsertLargeT
UseTagsExpressionYeastMutationPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-C–SaKKH-Cas9(D10A)
Plasmid#157840Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseCRISPRTagsExpressionMutationPromoterAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only