We narrowed to 14,348 results for: TIM
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Plasmid#223593PurposeLentiviral expression of human CD28 with mCherry tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)
Plasmid#160140PurposeCMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFPDepositorInserthuman codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsNLS(nucleoplasmin)-3xHA-P2A-EGFPExpressionMammalianPromoterCMV and T7Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CMV-Cas9-P2A-HygR
Plasmid#164133PurposeLentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cellsDepositorAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
VAMP8-mCherry
Plasmid#92424PurposeExpression of VAMP8 C-terminally conjugated to mCherry.DepositorInsertVAMP8 (Vamp8 Mouse)
TagsmCherryExpressionMammalianMutationA36S (Please see Depositor Comments below)PromoterCMVAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-FL
Plasmid#190821PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags6x HisExpressionBacterialMutationCodon optimized for expression in E.coliPromoterT7Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-tdTomato
Plasmid#199250PurposeFluorescent labelling of bacterial cells with tdTomatoDepositorInsertCodon-optimized tdTomato gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet
Plasmid#104006PurposePlasmid encoding mScarletDepositorInsertmScarlet
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC
Plasmid#183790PurposeA single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.DepositorInsertsP2A-eGFP-TargetBC-5xTAPE-1
pegRNA-InsertBC
UseSynthetic BiologyMutationG>A in the gRNA scaffold- please see depositor…Available SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-GCaMP7f
Plasmid#135539PurposeEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7fDepositorInsertXph20
TagsGCaMP7fExpressionMammalianPromoterCAGAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PKMYT1 gRNA (BRDN0001148430)
Plasmid#77279Purpose3rd generation lentiviral gRNA plasmid targeting human PKMYT1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)
Plasmid#133788PurposeCAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized St3Cas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mCherry
Plasmid#199247PurposeFluorescent labelling of bacterial cells with mCherryDepositorInsertCodon-optimized mCherry gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
enIscB-T5E
Plasmid#205411PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
ExpressionMammalianMutationE85R+H369R+S387R+S457RPromoterCAG, hU6, CMVAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES proHB-EGF WT
Plasmid#11602DepositorInsertHB-EGF (HBEGF Human)
ExpressionMammalianAvailable SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mKate2
Plasmid#199249PurposeFluorescent labelling of bacterial cells with mKate2DepositorInsertCodon-optimized mKate2 gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX458-GFP-TRIM28
Plasmid#185010PurposeEncodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFPDepositorInsertTRIM28 sgRNA (Trim28 Mouse)
UseCRISPRAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC1_oROS-HT
Plasmid#216413PurposeExpresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.DepositorInsertoROS-HT
ExpressionMammalianPromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only