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We narrowed to 9,297 results for: ef1

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Showing: 4401 - 4420 of 9297 results

  5. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  12. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175508
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175508-plasmid-map-sequence-id-345097

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  16. pCSN075

    Plasmid
    #166732
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166732-plasmid-map-sequence-id-332206

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  20. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid
    #188523
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Article
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Insert
    3XNLS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188523-plasmid-map-sequence-id-372834

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Showing: 4401 - 4420 of 9297 results