We narrowed to 39,129 results for: Eras

1. lentiMutB3GNT5-blast

   Plasmid

   #208380

   Purpose

   lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nt 922 c->g (PAM site inactivation)

   Promoter

   EF-1 alpha core promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFlag-Dam

   Plasmid

   #121909

   Purpose

   Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   Dam human codon optimized (dam E. coli)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-GGPPipi

   Plasmid

   #53280

   Purpose

   Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   crtE, idi
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2_Renilla_luc-60A

   Plasmid

   #200397

   Purpose

   A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-p300 reporter

   Plasmid

   #11726

   Depositor

   Eileen Adamson

   Article

   Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

   Insert

   p300 promoter and 5' UTR (EP300 )
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-RosaL6 ELD mutations

   Plasmid

   #37198

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Insert

   FokI (fused to Rosa L6 ZFN)
   

   Use

   Zfn expression

   Tags

   HA, ROSA26 eZFNs L6, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q486E, I499L and N496D

   Promoter

   CMV

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL-SUV39H2-L

   Plasmid

   #89136

   Purpose

   mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   SUV39H2 (SUV39H2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3xFLAG-CMV10-hAtg101

   Plasmid

   #22877

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

   Insert

   Atg13-interacting protein (ATG101 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

9. psicheck-2-Acsm2

   Plasmid

   #48158

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of Acsm2 (Acsm2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL2620 (pTniQ_Vch)

    Plasmid

    #200892

    Purpose

    Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTniQ
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE1062

    Plasmid

    #172356

    Purpose

    Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

    Depositor

    Eriko Takano

    Article

    Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

    Inserts

    scbR (SCO6265 )
    kasOp
    

    Use

    Synthetic Biology; Integrative into streptomyces …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIS3

    Plasmid

    #92173

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPbuCas13b

    Plasmid

    #184565

    Purpose

    Inducible expression of PbuCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Prevotella buccae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5 Myc-RIP1

    Plasmid

    #44159

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    RIP1 (RIPK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. P305-FBP_4

    Plasmid

    #139695

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDV5 containing Sb-convex

    Plasmid

    #132696

    Purpose

    pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_convex including 5' and 3' flanking sequences used for ribosome display
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCit(S1-S3)3-SAH

    Plasmid

    #228067

    Purpose

    Mammalian expression of coiled coil segments (S1-S3)x3, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCitrine, S1, S3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TagBFP(S2)3(S4)3-gs

    Plasmid

    #228068

    Purpose

    Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, S2, S4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSplit-CO-hDib1

    Plasmid

    #58788

    Purpose

    Express LucC-hDib1 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGrDL_SP

    Plasmid

    #83204

    Purpose

    receives miRNA target sequences for testing using the dual luciferase assay system

    Depositor

    Peer Schenk

    Article

    Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

    Insert

    SalI/PstI cloning sites
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. mEm-CoilE-Actin

    Plasmid

    #181993

    Purpose

    CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    mEm-CoilE-Actin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pPRIME-CMV-LNGFR-recipient

    Plasmid

    #11660

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    LNGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSUPER IKKe

    Plasmid

    #26211

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    pSUPER IKK epsilon (IKBKE Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGL3-Gateway-DSCP

    Plasmid

    #71506

    Purpose

    flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCDNA3.1-H-Luc_mito

    Plasmid

    #113930

    Purpose

    Plasmid encoding the mictochonfrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc_mito
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. tol2-CAG::ChR2-YFP

    Plasmid

    #59740

    Purpose

    Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    Channelrhodopsin-2
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CAG

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pSN444

    Plasmid

    #108331

    Purpose

    Expresses Sat2 for Nourseothricin resistance selection of C. elegans

    Depositor

    Shinsuke Niwa

    Article

    Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

    Insert

    Streptothricin acetyl transferase 2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-27 promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSPlit-OC-hDib1

    Plasmid

    #58789

    Purpose

    Express hDib1-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pCMV3TAG8li-hSTT3A-3FLAG

    Plasmid

    #159140

    Purpose

    Hu STT3A -flag mammalian expression vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    STT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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