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Mito-B

Plasmid

#209864

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

Depositor

Sarah Cohen

Publication

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange

Plasmid

#177827

Purpose

Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Publication

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange

Expression

Mammalian

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Publication

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Expression

Mammalian

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRpHLuorin2-N1

Plasmid

#171716

Purpose

Ratiometric pHLuorin2 backbone (C terminal fusion)

Depositor

Geert van den Bogaart

Publication

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Type

Empty backbone

Expression

Mammalian

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ER-RpHLuorin2

Plasmid

#171717

Purpose

Luminal ER expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Publication

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 targeted to ER

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pKRaION2-N1

Plasmid

#177802

Purpose

Mammalian expression of green fluorescent potassium indicator KRaION2

Depositor

Edward Boyden

Publication

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Publication

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Publication

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pNHEJ-recX-sacB

Plasmid

#158718

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Expression

Bacterial

Promoter

Pmyc

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry- eMagBF - 5ptaseOCRL

Plasmid

#162254

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Publication

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

mCherry- eMagBF - 5ptaseOCRL

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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piGECI-N1

Plasmid

#160421

Purpose

Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Publication

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3b

Plasmid

#111056

Purpose

Expresses dLight1.3b in mammalian cells

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3b

Tags

Flag tag

Expression

Mammalian

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpyTag003-mClover3

Plasmid

#133453

Purpose

Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mClover3

Tags

GSGSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMKV060

Plasmid

#133315

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+

Expression

Plant

Promoter

AtUbi10

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMC037-HisMS2_PLP_pac

Plasmid

#128233

Purpose

Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Publication

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Publication

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221_SLC16A3

Plasmid

#131899

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A3 (SLC16A3 Human)

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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COX8-SuperNova

Plasmid

#131856

Purpose

Expresses SuperNova fused to mitochrondrial protein COX8A

Depositor

Geert van den Bogaart

Publication

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

Cox8A and Myc tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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