We narrowed to 88,342 results for: lias

1. mCherry-eDHFR-Cdc42Q61L

   Plasmid

   #107267

   Purpose

   mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX307 DEDD

   Plasmid

   #117744

   Purpose

   Open reading frame vector encoding DEDD

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   DEDD1 (DEDD Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 139H2_HC

   Plasmid

   #206201

   Purpose

   For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

   Depositor

   Joost Snijder

   Article

   Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

   Insert

   anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
   

   Use

   Tags

   -AAAHHHHHHHH

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

   Plasmid

   #176061

   Purpose

   Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-GFP-ATL1-G20TM

   Plasmid

   #109019

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL1-deltaTM (ATL1 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   truncated form without C-terminal ER transmembran…

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CreLite

   Plasmid

   #131785

   Purpose

   AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC-P2A-PIF6CreN
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-NLS-gs10-cDHFR174

   Plasmid

   #188256

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cDHFR174 (DHFR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Puro Cas13d P2A eGFP-NLS

   Plasmid

   #155182

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   CasRx
   

   Use

   Unspecified; Piggybac transposon

   Tags

   NLS and P2A eGFP NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS2260

    Plasmid

    #158423

    Purpose

    AAV plasmid with Ple331 (PAX6 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple331 (PAX6 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple331

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4 3xFLAG Halo delta 118-180 nsp1

    Plasmid

    #175424

    Purpose

    Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 118-180 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminus of nsp1

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe JunD-Venus puro

    Plasmid

    #58496

    Purpose

    mammalian expression of Venus-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTE4565

    Plasmid

    #88903

    Purpose

    Expresses inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pE-cadherin-GFP-N-WASP deltaVCA

    Plasmid

    #101283

    Purpose

    Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    delta VCA domain

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGK-hMIGA2-DsRed

    Plasmid

    #192867

    Purpose

    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTE4567

    Plasmid

    #107557

    Purpose

    Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(dead2)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    E1080A

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNAM-human eIF2C1-WT-Myc

    Plasmid

    #50360

    Purpose

    Expression of human eIF2C1 in mamalian cells

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C1 (AGO1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only