We narrowed to 6,928 results for: 11

1. pCDNA3.1-nAChr-beta3

   Plasmid

   #48811

   Purpose

   This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

   Depositor

   Henry Lester, Jerry Stitzel

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRR.6

   Plasmid

   #69997

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6 variant

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q172H, K262R

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RBM6_RRM_pGEX

   Plasmid

   #135157

   Purpose

   Recombinant expression of RBM6 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBM6 (RBM6 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. His 3C Kinase dead MKK6 1-334

   Plasmid

   #87882

   Purpose

   E. coli expression of MKK6 kinase dead

   Depositor

   Peter Mace

   Article

   Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   3C protease site and 6xHis

   Expression

   Bacterial

   Mutation

   K82A, kinase dead mutant

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pECE beta1 R760A

   Plasmid

   #50511

   Depositor

   Kenneth Yamada

   Article

   Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

   Insert

   integrin beta1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R760A

   Promoter

   SV40

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMGF157

   Plasmid

   #96972

   Purpose

   ish1-mCherry insertion cassette with Hygro resistance gene

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + hygro…

   Promoter

   N/A

   Available since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. integrin beta6 777T pBabe puro

   Plasmid

   #13583

   Depositor

   Dean Sheppard

   Article

   Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

   Insert

   integrin beta 6 777T (ITGB6 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   777T. Lacks last 11 amino acids of cytoplasmic do…

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pRR-S1

   Plasmid

   #69998

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6 variant

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G329T (G110V)

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRR-S2

   Plasmid

   #69999

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6 variant

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T441C (I114T)

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRR-S4

    Plasmid

    #70000

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6 variant

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G835C (A279P)

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRR-S5

    Plasmid

    #70001

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6 variant

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T548G (V183G)

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRR-S6a

    Plasmid

    #70002

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6 variant

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T637C (F213L)

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRR-S7

    Plasmid

    #70003

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6 variant

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G758A (R253H)

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRR-S8

    Plasmid

    #70004

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6 variant

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C1459A (R487S)

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMGF170

    Plasmid

    #96997

    Purpose

    ish1-mCherry insertion cassette with NAT resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + NAT r…

    Promoter

    N/A

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-PRAS40 F129A

    Plasmid

    #86763

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human PRAS40 (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F129A

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-PRAS40 W10A

    Plasmid

    #86761

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human PRAS40 (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    W10A

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-10-3xFLAG-LGP2-MIII

    Plasmid

    #58683

    Purpose

    Expresses LGP2 with a mutation to motif III in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

    Insert

    LGP2-MIII (DHX58 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    threonine and serine of helicase motif III mutate…

    Promoter

    CMV

    Available since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. 2.3 hA33 luciferase

    Plasmid

    #68148

    Purpose

    luciferase reporter for hA33 promoter

    Depositor

    Joan Heath

    Article

    Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

    Insert

    A33 (GPA33 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    starts at -2270 relative to ATG

    Promoter

    human GPA33 promoter

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Gal4-VP16-HSF1Δ∼PRD

    Plasmid

    #71727

    Purpose

    plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Budding Yeast, Herpes Simplex Virus protein VP16, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.BRWD3-V5H

    Plasmid

    #48624

    Purpose

    Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    BRWD3 (BRWD3 Fly)
    

    Use

    Tags

    V5 and His tags

    Expression

    Mammalian

    Mutation

    no mutations, stop was removed to fuse V5His at C…

    Promoter

    CMV

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

22. AAV-DIO- alpha-MSH

    Plasmid

    #239044

    Purpose

    Overexpression of alpha-MSH

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    alpha-MSH (Pomc Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-DIO-beta-Endorphin

    Plasmid

    #239043

    Purpose

    Overexpression of beta endorphin

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    beta-Endorphin (Pomc Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-DIO-mPOMC

    Plasmid

    #239042

    Purpose

    Overexpression of POMC gene

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    mPOMC (Pomc Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT-hMAPT

    Plasmid

    #231583

    Purpose

    Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

    Insert

    human MAPT (MAPT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5 FRT TO GFP11-LRRK2

    Plasmid

    #231174

    Purpose

    Expresses GFP-11-LRRK2 (wildtype, full length) in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 10279-M30-663

    Plasmid

    #225663

    Purpose

    Lentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholding

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mCMVp

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCA528 CHMP6 145-201

    Plasmid

    #104597

    Purpose

    Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP6 residues 145-201 (CHMP6 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pACB112

    Plasmid

    #222211

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(S11R)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Changed Serine 11 to Arginine

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid

    #222876

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FRB chain: V84R in GPA

    Promoter

    MSCV LTR

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge