We narrowed to 27,276 results for: Tat

1. pADSS-EGFP

   Plasmid

   #228197

   Purpose

   expressed human ADSS with an EGFP fluorescent protein tag

   Depositor

   Stephen Benkovic

   Article

   Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

   Insert

   ADSS (ADSS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pB-3xFlag-dCas13-MBD6MBD

   Plasmid

   #228233

   Purpose

   For targeted tethering of the MBD domain of human MBD6 using dCas13

   Depositor

   Chuan He

   Article

   Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

   Inserts

   dCas13
   MBD6 (MBD6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

   Plasmid

   #226884

   Purpose

   Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

   Depositor

   Ning Zheng

   Article

   Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

   Insert

   NaVAb
   

   Use

   Tags

   MBP

   Expression

   Insect

   Mutation

   R4A, N49K, L109A, M116V

   Promoter

   Polyhedrin

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJExpress401-KDAC8 D101R

   Plasmid

   #224349

   Purpose

   Expresses human KDAC8 (HDAC8) D101R in E. coli

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

   Insert

   KDAC8 (HDAC8 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Bacterial

   Mutation

   Aspartate 101 mutated to arginine

   Promoter

   T5

   Available since

   Oct. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJExpress401-KDAC8 D101A

   Plasmid

   #224347

   Purpose

   Expresses human KDAC8 (HDAC8) D101A in E. coli

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

   Insert

   KDAC8 (HDAC8 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Bacterial

   Mutation

   Aspartate 101 mutated to alanine

   Promoter

   T5

   Available since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pU6-hL1-5'_3.3kb plasmid

   Plasmid

   #226006

   Purpose

   Plasmid used to generate DNA-FISH probe targeting at human L1HS

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   Human L1 element 3.3kb sequences at the 5' end
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3-Ctr1-gRNA-EGFP

   Plasmid

   #226000

   Purpose

   Negative control for CRISPRi-KD

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   negtive control sgRNA of no specific targets in human genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-Ctr2-gRNA-EGFP

   Plasmid

   #226001

   Purpose

   Negative control for CRISPRi-KD

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   negtive control sgRNA of no specific targets in human genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-NCL-gRNA1-EGFP

   Plasmid

   #226004

   Purpose

   CRISPRi-KD of human Nucleolin

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   sgRNA targeting human Nucleolin (NCL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-NCL-gRNA2-EGFP

    Plasmid

    #226005

    Purpose

    CRISPRi-KD of human Nucleolin

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    sgRNA targeting human Nucleolin (NCL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiCHECK-1-miR-455-WT

    Plasmid

    #215881

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psiCHECK-1-miR-376a1(3p)-I-type

    Plasmid

    #215883

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. psiCHECK-1-miR-589(5p)-I-type

    Plasmid

    #215884

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psiCHECK-1-miR-589(3p)-I-type

    Plasmid

    #215885

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-PODXL S481A

    Plasmid

    #195223

    Purpose

    Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S481A

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWZL-hygro-FAKS722A

    Plasmid

    #216545

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: S722A) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (S722A) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation S722A

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWZL-hygro-FAKY861F

    Plasmid

    #216546

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: Y861F) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (Y861F) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation Y861F

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWZL-hygro-FAKY397F

    Plasmid

    #216677

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: Y397F) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (Y397F) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation Y397F

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWZL-hygro-FAKS732A

    Plasmid

    #216541

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (S732A) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation S732A

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWZL-hygro-FAKS910A

    Plasmid

    #216542

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (S910A) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation S910A

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-EF1a-ArgiNLS-miRFP670

    Plasmid

    #220600

    Purpose

    Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    miRFP670
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-EF1a-ArgiNLS-AausFP1

    Plasmid

    #220595

    Purpose

    Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

    Plasmid

    #216171

    Purpose

    Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRAR107

    Plasmid

    #185037

    Purpose

    400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AGA74-76GAG mutation of rpmE 5'UTR, Only fir…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PX458-SPNS1-sg1

    Plasmid

    #198566

    Purpose

    SPNS1-targeting sgRNA in PX458 vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1-sg1 (SPNS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

    Plasmid

    #131683

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    EF1a and mU6

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCPH2A.X_K5Q-GS3

    Plasmid

    #204746

    Purpose

    Expression of Cas9 and human H2AX with the K5Q mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K5Q (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K5Q mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCPH2A.X_K5R-GS3

    Plasmid

    #204747

    Purpose

    Expression of Cas9 and human H2AX with the K5R mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K5R (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K5R mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCPH2A.X_K134L-GS3

    Plasmid

    #204750

    Purpose

    Expression of Cas9 and human H2AX with the K134L mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134L (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134L mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCPH2A.X_K134M-GS3

    Plasmid

    #204751

    Purpose

    Expression of Cas9 and human H2AX with the K134M mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134M (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134M mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCPH2A.X_K134A-GS3

    Plasmid

    #204752

    Purpose

    Expression of Cas9 and human H2AX with the K134A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134A (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134A mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. lenti-KRAS-G121C/G13A-nick sgRNA

    Plasmid

    #214099

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    KRAS G12C or G13A nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CMV-IgK-pHluorin-TM-mRuby

    Plasmid

    #185546

    Purpose

    Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    IgK-pHluorin-TM-mRuby
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AA233

    Plasmid

    #215941

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Dyn1 R271E D291R

    Plasmid

    #198342

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Dyn1 R271E

    Plasmid

    #198343

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Dyn1 R271E E720R

    Plasmid

    #198344

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Dyn1 K44A

    Plasmid

    #198340

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Dyn1 D406A

    Plasmid

    #198341

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406A

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge