We narrowed to 10,848 results for: ENA

1. Bxb1_miRFP703

   Plasmid

   #183759

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   miRFP703
   

   Use

   Tags

   Lck lipidation signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC_C_LAMP1

   Plasmid

   #111669

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIRECT_26H

   Plasmid

   #91150

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEMS-SDHB-Halo7Tag

   Plasmid

   #108973

   Purpose

   Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

   Depositor

   Karin Busch

   Article

   Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

   Insert

   succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
   

   Use

   Tags

   Halo7-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PA-mCherry1-Vimentin-7

   Plasmid

   #57202

   Purpose

   Localization: Intermediate Filaments, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ATG9A-2STrep

   Plasmid

   #210359

   Purpose

   Expresses ATG9A fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   ATG9A (ATG9A Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_B2515b

   Plasmid

   #91073

   Purpose

   Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_C2616

   Plasmid

   #91090

   Purpose

   Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   At7SL

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs_FLAG-MCART2

   Plasmid

   #133250

   Purpose

   Express FLAG-tagged MCART2 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   MCART2 (SLC25A52 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMOD_B2301

    Plasmid

    #91067

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

    Plasmid

    #61411

    Purpose

    Expresses human OTUD3 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 276-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINK-OTUD5 (OTU, aa 171-358)

    Plasmid

    #61415

    Purpose

    Expresses human OTUD5 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-170 and aa 359-571.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pASPIre1

    Plasmid

    #154842

    Purpose

    Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

    Insert

    Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Prha

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs_FLAG-NDT1

    Plasmid

    #133254

    Purpose

    Express FLAG-tagged NDT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT1 (YIA6 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti CRISPR sgMTHFD2_1

    Plasmid

    #106313

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2 (MTHFD2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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