We narrowed to 43,284 results for: cha;

1. NFAST(low)-mApple-mito

   Plasmid

   #214415

   Purpose

   Mitochondria localization of low-affinity NFAST fused to mApple-mito

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspA-N
   

   Use

   Tags

   mApple-mito

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pANAP

   Plasmid

   #48696

   Purpose

   Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

   Depositor

   Peter Schultz

   Article

   Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

   Inserts

   8 copies of the amber suppressor tRNACUA-Leu
   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant E.coli leucyl synthetase specific for Anap…

   Promoter

   CMV and human H1 promoter

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGMCtKq28-TSC10M1-sspB

   Plasmid

   #49526

   Purpose

   Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   TSC10M1-sspB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

   Plasmid

   #31481

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce target) EF BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry contains M9S and M16L to reduce backgroun…

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CAG-mNeonGreen

   Plasmid

   #99134

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-P2X2-YFP

   Plasmid

   #22400

   Depositor

   Henry Lester

   Article

   Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

   Insert

   ATP-gated P2X channel 2 (P2rx2 Rat)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CapChR2_pmScarlet-N1

   Plasmid

   #188032

   Purpose

   Expresses CapChR2-mScarlet in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDH-EF1-FHC-POLQ

   Plasmid

   #64875

   Purpose

   lentiviral expression of human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. plentiCMV-sp-HPV-TM(ICAM)

   Plasmid

   #196510

   Purpose

   Express HPV antigen epitope with ICAM transmembrane domain

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   a HPV epitope with CD8a stalk linker and ICAM TM domain
   

   Use

   Lentiviral

   Tags

   3xflag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEC3006 (pSpy1C-PZn_ffluc)

    Plasmid

    #218515

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PZn (PczcD)

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLR16-Phes*

    Plasmid

    #98783

    Purpose

    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

    Depositor

    Anat Herskovits

    Article

    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

    Insert

    pheS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A309G

    Promoter

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid

    #193323

    Purpose

    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Depositor

    Michael Lin

    Article

    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

    Insert

    ASAP3-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

13. pFsynW SYT1 reporter

    Plasmid

    #197275

    Purpose

    Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-FoxO1-Clover

    Plasmid

    #67759

    Purpose

    Fluorescent fusion protein for tracking Akt signaling

    Depositor

    Peter Rotwein

    Article

    Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

    Insert

    Fox01 (Foxo1 Mouse)
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mutation

    Changed Serine 209 to Alanine, changed Histidine …

    Promoter

    EF1a

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-mTurquoise2

    Plasmid

    #99122

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGMCtN-TSC10M1-sspB

    Plasmid

    #49521

    Purpose

    Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    TSC10M1-sspB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid

    #137827

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAVS1-Puro cTnT FUCCI

    Plasmid

    #136935

    Purpose

    donor plasmid for cardiac-specific FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Troponin T promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cTnT

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pREDTAI

    Plasmid

    #51627

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGPA1

    Plasmid

    #115476

    Purpose

    Generation of mariner-based transposon mutant libraries in Enterococcus faecium

    Depositor

    Willem van Schaik

    Article

    Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Mfn1-Myc

    Plasmid

    #23212

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn1 (Mfn1 Mouse)
    

    Use

    Tags

    6xHis, 9xMyc, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGL2-NOS2Promoter-Luciferase

    Plasmid

    #19296

    Depositor

    Charles Lowenstein

    Article

    Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

    Insert

    NOS2 Promoter (Nos2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMVTNT-T7-KPNA1

    Plasmid

    #26677

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 . 11():63.

    Insert

    Karyopherin Alpha 1 (KPNA1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    KPNA1 sequence contains a reported SNP at S73N

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21-pelB-GST-4

    Plasmid

    #228986

    Purpose

    For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-4
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21-pelB-GST-3

    Plasmid

    #228985

    Purpose

    For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-3
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-mCherry-MHC-IIA

    Plasmid

    #35687

    Depositor

    Venkaiah Betapudi

    Article

    Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

    Insert

    MHC IIA (MYH9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. UPB-colon (UPB4)

    Plasmid

    #180786

    Purpose

    Unique Projection Barcode (UPB4)-Colon for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial )
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn1-mNeonGreen

    Plasmid

    #99135

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NAX_CAGGS_CAS9_m4_KRAB_mcherry

    Plasmid

    #167878

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

34. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 3122 pSFFV-neo Bcl-XL MI-3

    Plasmid

    #8811

    Depositor

    Stanley Korsmeyer

    Article

    Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

    Insert

    Bcl-XL MI-3 (BCL2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    BH1 WGR-->WAR

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

37. pETDuet1_His-TEV-EGFP

    Plasmid

    #223723

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    EGFP, not monomeric (has A207)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJL1-sfGFP-DQNAT

    Plasmid

    #198210

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pλPR cI-Elysis

    Plasmid

    #176891

    Purpose

    Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

    Depositor

    James Inglese

    Article

    Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

    Insert

    Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge