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Showing: 11151 - 11200 of 12598 results

  1. CCRL2-DuET

    Plasmid
    #213205
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCRL2 (CCRL2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213205-plasmid-map-sequence-id-430768

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  2. C5A-DuET

    Plasmid
    #213192
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    C5A (C5AR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213192-plasmid-map-sequence-id-430757

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  3. CCKAR-DuET

    Plasmid
    #213195
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCKAR (CCKAR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213195-plasmid-map-sequence-id-430759

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  4. CCKBR-DuET

    Plasmid
    #213196
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCKBR (CCKBR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213196-plasmid-map-sequence-id-430760

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  5. AVPR1B-DuET

    Plasmid
    #213187
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR1B (AVPR1B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213187-plasmid-map-sequence-id-430752

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  6. AVPR2-DuET

    Plasmid
    #213188
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR2 (AVPR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213188-plasmid-map-sequence-id-430753

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  7. ADRA2C-DuET

    Plasmid
    #213179
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRA2C (ADRA2C Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213179-plasmid-map-sequence-id-430746

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  22. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194890
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    194890-plasmid-map-sequence-id-392583

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  23. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194895
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194895-plasmid-map-sequence-id-389971

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  29. pBetaActin-FKBP-Halo-Dync1h1motor

    Plasmid
    #191333
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191333-plasmid-map-sequence-id-381754

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  31. pGADT7-ε

    Plasmid
    #197261
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-4 ε (AP4E1 Human)
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitutions in codons 905, 1129 and 1137
    Promoter
    ADH1
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197261-plasmid-map-sequence-id-387043

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  33. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194889
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194889-plasmid-map-sequence-id-382866

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  34. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194891
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194891-plasmid-map-sequence-id-382865

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  35. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

    Plasmid
    #194896
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_R396A
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194896-plasmid-map-sequence-id-382861

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  36. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

    Plasmid
    #194888
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_R396A
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194888-plasmid-map-sequence-id-382857

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  37. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194887
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194887-plasmid-map-sequence-id-382813

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  38. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194893
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194893-plasmid-map-sequence-id-382802

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  39. pcDNA3.1_Tau Authentic Alu 10-12WT

    Plasmid
    #194167
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 WT
    Tags
    3X Flag
    Expression
    Mammalian
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194167-plasmid-map-sequence-id-381798

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  40. pcDNA3.1_Tau Authentic Alu 7-12WT

    Plasmid
    #194170
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    microtubule-associated protein tau 7-12 WT
    Tags
    3x Flag
    Expression
    Mammalian
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194170-plasmid-map-sequence-id-381818

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  41. pcDNA3.1_ZKSCAN1_Tau10-12V337M

    Plasmid
    #194165
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau V337M mutation. Expresses the tau circRNA 12-->10 V337M with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 V337M
    Expression
    Mammalian
    Mutation
    Changed Valine 337 to Methinonine
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194165-plasmid-map-sequence-id-381806

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Showing: 11151 - 11200 of 12598 results