Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,157 results for: pcDNA

Clear keywords
Showing: 11151 - 11200 of 12157 results

  10. C211

    Plasmid
    #174360
    Purpose
    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection
    Depositor
    David Bartel
    Article
    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1
    Insert
    PABPC1 (PABPC1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …
    Available Since
    Feb. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    174360-plasmid-map-sequence-id-343304

    Enlarge

  12. pRD397

    Plasmid
    #163104
    Purpose
    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mTurquoise2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163104-plasmid-map-sequence-id-321845

    Enlarge

  21. Myc-delta/gamma2SIL

    Plasmid
    #119731
    Purpose
    GABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRD/GABRG2 (Gabrg2, Gabrd Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    119731-plasmid-map-sequence-id-229623

    Enlarge

  22. G protein alpha-o-R179C-EE

    Plasmid
    #55780
    Purpose
    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Bacterial and Mammalian
    Mutation
    Arginine 179 was replaced with cysteine
    Promoter
    CMV
    Available Since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    55780-plasmid-map-sequence-id-122021

    Enlarge

  36. sgFFL_3x_siRNA

    Plasmid
    #59893
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has three siRNA-like targets (fully c…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59893-plasmid-map-sequence-id-108250

    Enlarge

  43. huCofilin S3E SSR

    Plasmid
    #245940
    Purpose
    Expresses human cofilin with mutation S3E that behaves like inactive phosphocofilin but can be dominant negative against phosphatase
    Depositor
    James Bamburg
    Article
    Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7
    Insert
    cofilin 1 (cfl1) (CFL1 Human)
    Expression
    Mammalian
    Mutation
    S3E; silent mutations (NTs 67 to 75) in which TCT…
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    245940-plasmid-map-sequence-id-490033

    Enlarge

  44. pSmBtA3-d7F

    Plasmid
    #245784
    Purpose
    Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells
    Depositor
    Vsevolod Gurevich
    Article
    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3
    Insert
    arrestin-3 (ARR3 Bovine)
    Tags
    Flag and SmBiT
    Expression
    Mammalian
    Mutation
    deleted residues 175, 177, 178, 182-185
    Promoter
    CMV
    Available Since
    Oct. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    245784-plasmid-map-sequence-id-488534

    Enlarge

  47. pVBL0001

    Plasmid
    #242429
    Purpose
    Stable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.
    Depositor
    Vladislav Belyy
    Article
    Smith et al bioRxiv [Preprint]. 2025 Jul 21:2025.07.
    Insert
    IRE1alpha (ERN1 Human)
    Tags
    mCherry, CRY2clust
    Expression
    Mammalian
    Mutation
    Deletion of the lumenal domain
    Promoter
    CMV, tet-inducible
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242429-plasmid-map-sequence-id-484493

    Enlarge

Showing: 11151 - 11200 of 12157 results