We narrowed to 11,735 results for: pcDNA
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Plasmid#135381PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 )DepositorInsertnSaCas9-ABEMax(V82G) H119
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 M732 miniABEMax V82G
Plasmid#135382PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )DepositorInsertnSaCas9 M732 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 M741 miniABEMax V82G
Plasmid#135383PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )DepositorInsertnSaCas9 M741 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 N120 miniABEMax V82G
Plasmid#135384PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )DepositorInsertnSaCas9 N120 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 AIDx no UGI
Plasmid#135349PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.DepositorInsertnSpyCas9-hAIDx-ID-1058
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 APOBEC3 no UGI
Plasmid#135350PurposeExpression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.DepositorInsertnSpyCas9-rAPOBEC3-ID-1058
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 AIDx with UGI
Plasmid#135354PurposeExpression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminusDepositorInserthAIDx-nSpyCas9 aa-UGI
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 ABEMax
Plasmid#135356PurposeExpression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9DepositorInsertnSpyCas9-ABEMax aa 1058
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 3
Plasmid#135359PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminusDepositorInsert1029 CP nSpyCas9 ABEMax
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 miniABEMax V82G strategy1
Plasmid#135360PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminusDepositorInsertmini ABEMax 1029 nSpyCas9
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 V82G ABEMax
Plasmid#135361PurposeExpression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9DepositorInsert1058 nSpyCas9 V82G ABEMax
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 V82G ABEMax Strategy 3
Plasmid#135363PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminusDepositorInsert1029 V82G ABEMax
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniABEMax V82G nSaCas9
Plasmid#135365PurposeExpression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitutionDepositorInsertminiABEMax V82G nSaCas9
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-202
Plasmid#135344PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editingDepositorInsertnSpyCas9-hAIDx-ID-202
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXD11-Adel-IDR
Plasmid#145241PurposeExpresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDRDepositorInsertHOXD11-Adel-IDR (HOXD11 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13+7A-IDR
Plasmid#145239PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDRDepositorInsertHOXA13+7A-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HEY2wt-IDR
Plasmid#145235PurposeExpresses fusion of GAL4 DNA-binding domain and HEY2wt IDRDepositorInsertHEY2wt-IDR (HEY2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-GSX2wt-IDR
Plasmid#145233PurposeExpresses fusion of GAL4 DNA-binding domain and GSX2wt IDRDepositorInsertGSX2wt-IDR (GSX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-EOMES-Adel-IDR
Plasmid#145230PurposeExpresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDRDepositorInsertEOMES-Adel-IDR (EOMES Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXD13wt-IDR
Plasmid#145244PurposeExpresses fusion of GAL4 DNA-binding domain and HOXD13wt IDRDepositorInsertHOXD13wt-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-GSX2-Adel-IDR
Plasmid#145232PurposeExpresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDRDepositorInsertGSX2-Adel-IDR (GSX2 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Mm_Plk5(300-595) PBD domain
Plasmid#136316PurposeMammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFPDepositorInsertPLK5 (Plk5 Mouse)
UseTagsExpressionMammalianMutationfragment 300-595PromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SENP2 NPm
Plasmid#126593PurposeExpresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell linesDepositorInsertSENP2 (SENP2 Human)
UseTagsFLAGExpressionMammalianMutationDeletion of amino acids 1-65 and L329A/L331A in t…PromoterCMVAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SENP2 CCm
Plasmid#126594PurposeExpresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell linesDepositorInsertSENP2 (SENP2 Human)
UseTagsFLAGExpressionMammalianMutationDeletion of amino acids 203-228 and siRNA resista…PromoterCMVAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL405 CHYRON1 integration construct
Plasmid#126442PurposeTo integrate the CHYRON1 locus at HEK293site3.DepositorInsertspU6-CHYRON1 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR149
Plasmid#106049PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)DepositorInsertOpto-GPR149 (GPR149 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR153
Plasmid#106053PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR153; Opto-GPR153; E7)DepositorInsertOpto-GPR153 (GPR153 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR160
Plasmid#106054PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR160; Opto-GPR160; E8)DepositorInsertOpto-GPR160 (GPR160 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR162
Plasmid#106056PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR162; Opto-GPR162; E10)DepositorInsertOpto-GPR162 (GPR162 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR171
Plasmid#106057PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR171; Opto-GPR171; E11)DepositorInsertOpto-GPR171 (GPR171 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR174
Plasmid#106059PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR174; Opto-GPR174; F1)DepositorInsertOpto-GPR174 (GPR174 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR82
Plasmid#106034PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR82; Opto-GPR82; C12)DepositorInsertOpto-GPR82 (GPR82 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR83
Plasmid#106035PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR83; Opto-GPR83; D1)DepositorInsertOpto-GPR83 (GPR83 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR84
Plasmid#106036PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR84; Opto-GPR84; D2)DepositorInsertOpto-GPR84 (GPR84 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR85
Plasmid#106037PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR85; Opto-GPR85; D3)DepositorInsertOpto-GPR85 (GPR85 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR87
Plasmid#106038PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR87; Opto-GPR87; D4)DepositorInsertOpto-GPR87 (GPR87 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR120
Plasmid#106041PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR120; Opto-GPR120; D7)DepositorInsertOpto-GPR120 (FFAR4 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR142
Plasmid#106046PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR142; Opto-GPR142; D12)DepositorInsertOpto-GPR142 (GPR142 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR34
Plasmid#106017PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR34; Opto-GPR34; B7)DepositorInsertOpto-GPR34 (GPR34 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR45
Plasmid#106023PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR45; Opto-GPR45; C1)DepositorInsertOpto-GPR45 (GPR45 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR52
Plasmid#106025PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR52; Opto-GPR52; C3)DepositorInsertOpto-GPR52 (GPR52 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR63
Plasmid#106029PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR63; Opto-GPR63; C7)DepositorInsertOpto-GPR63 (GPR63 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR65
Plasmid#106030PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR65; Opto-GPR65; C8)DepositorInsertOpto-GPR65 (GPR65 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR68
Plasmid#106031PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR68; Opto-GPR68; C9)DepositorInsertOpto-GPR68 (GPR68 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR6
Plasmid#106002PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR6; Opto-GPR6; A4)DepositorInsertOpto-GPR6 (GPR6 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR20
Plasmid#106008PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR20; Opto-GPR20; A10)DepositorInsertOpto-GPR20 (GPR20 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR22
Plasmid#106010PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR22; Opto-GPR22; A12)DepositorInsertOpto-GPR22 (GPR22 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR26
Plasmid#106012PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR26; Opto-GPR26; B2)DepositorInsertOpto-GPR26 (GPR26 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE3314
Plasmid#107530PurposeExpresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.DepositorInserthFnCpf1(RVR)
UseTags3xHA and NLSExpressionMammalianMutationN607R, K613V, N617RPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE3319
Plasmid#107533PurposeExpresses human codon optimized MbCpf1(RR mutant) in mammalian cells.DepositorInserthMbCpf1(RR)
UseTags3xHA and NLSExpressionMammalianMutationN576R, K637RPromoterCMVAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only