We narrowed to 23,455 results for: Spr

1. pX1-IFl_Con-IFr

   Plasmid

   #192216

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X1)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX2-IFl_Con-IFr

   Plasmid

   #192217

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUt-mNF-Cas13d

   Plasmid

   #224789

   Purpose

   LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   mCherry-P2A-RfxCas13d
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-d2i

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMV_AA091

   Plasmid

   #216106

   Purpose

   CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gRNA expression with modified trRNA to recruit activators via PP7 tags
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

   Plasmid

   #189633

   Purpose

   Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

   Plasmid

   #189632

   Purpose

   Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

   Plasmid

   #179072

   Purpose

   Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   TDH3p-Neon(gfp)-Link-2xPH(PLCd)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

   Plasmid

   #179038

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membrane

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

   Plasmid

   #157982

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDECKO_mCherry_NFE2

    Plasmid

    #140105

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDECKO_mCherry_TREML2

    Plasmid

    #140106

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDECKO_mCherry_LINC02432

    Plasmid

    #140107

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDECKO_mCherry_MIR3945HG

    Plasmid

    #140108

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO_mCherry_Intergenic

    Plasmid

    #140100

    Purpose

    CRISPR-Cas9 library validation (negative control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDECKO_mCherry_FURIN

    Plasmid

    #140103

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-sgALCAM

    Plasmid

    #83929

    Purpose

    Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgALCAM
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-sgSLC35B2

    Plasmid

    #83932

    Purpose

    Lentiviral vector expressing an sgRNA targeting SLC35B2 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgSLC35B2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-sgTPST2

    Plasmid

    #83933

    Purpose

    Lentiviral vector expressing an sgRNA targeting TPST2 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgTPST2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX459-sgPLXNB2

    Plasmid

    #86151

    Purpose

    Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pCMV_eA3A T31A-nCas9

    Plasmid

    #163538

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31A

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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