We narrowed to 11,743 results for: pcDNA

1. pcDNA3-Lyn-Akt-STOPS

   Plasmid

   #175009

   Purpose

   Plasma membrane-targeted Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   Lyn-mCherry-Akt-STOPS
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-MTOR-F1888L

   Plasmid

   #69010

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-F1888L (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Phenylalanine 1888 to Leucine

   Promoter

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

   Plasmid

   #145770

   Purpose

   expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Stop codon at Serine 2013

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3puro-mGFP-FXR1a-G266E

   Plasmid

   #225467

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a (G266E)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   G266E

   Promoter

   CMV

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-eYFP-FKBP-MAD1-WT

   Plasmid

   #114037

   Purpose

   Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

   Depositor

   Geert Kops

   Article

   Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

   Insert

   Human MAD1 (MAD1L1 Human)
   

   Use

   Tags

   eYFP-FKBP12

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA_SZ1-RGG-GFP-RGG

   Plasmid

   #124932

   Purpose

   Expression of SZ1-RGG-GFP-RGG

   Depositor

   Benjamin Schuster, Daniel Hammer, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 human cofilin S3E

   Plasmid

   #50855

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pcDNA3.3−3xFLAG−AGO2(S387A)

   Plasmid

   #220462

   Purpose

   Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3−3xFLAG−AGO2(S387D)

   Plasmid

   #220463

   Purpose

   Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to aspartate

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_Tau Authentic Alu 10-12V337M

    Plasmid

    #194169

    Purpose

    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 V337M
    

    Use

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Changed Valine 337 to Methinonine

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-FLAG-human NPM1-C1

    Plasmid

    #160693

    Purpose

    Mammalian expression of FLAG-tagged NPM1 C1 deletion mutant lacking nucleic acid-binding domain (NBD).

    Depositor

    Charles Sherr

    Article

    Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks NBD domain

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag MFF iso2 WT

    Plasmid

    #74389

    Purpose

    Flag tagged human MFF isoform 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3puro-mGFP-ROCK2-N (1-941)

    Plasmid

    #225473

    Purpose

    Express mEGFP-fusion protein of ROCK2 fragment (1-941)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N terminal fragment (1-941)

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.0_LynLAMA-F98 IRES EGFP

    Plasmid

    #130710

    Purpose

    Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP
    

    Use

    Tags

    Lyn11 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO HIS HSPA1L

    Plasmid

    #19538

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 1-like (HSPA1L Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1(+)-myc-SNAP-GP135

    Plasmid

    #50376

    Purpose

    Express myc and SNAP-tagged GP135 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5/FRT/TO GFP HSPA2

    Plasmid

    #19485

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 2 (HSPA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 6A CARD8 CARD-FLAG WT

    Plasmid

    #164020

    Purpose

    Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4-PKCZeta WT His tagged

    Plasmid

    #24609

    Depositor

    Jeff Wrana

    Article

    Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

    Insert

    PKCZeta (PRKCZ Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits