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p6350 MSCV-CMV-CMV-Flag-HA-ATAD5

Plasmid

#31356

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

ATAD5 (ATAD5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA

Plasmid

#113896

Purpose

Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Promoter

CMV-MIE-TetO2

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDarmo.CMVT_v1

Plasmid

#133072

Purpose

Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-Flag-TwinStrep and TwinStrep-Flag-3C

Expression

Mammalian

Promoter

CMVT (Enhanced CMV)

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTACR TH1-p2aGFP

Plasmid

#135815

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 1 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTACR TH2-p2aGFP

Plasmid

#135816

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 2 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Structural polyprotein

Plasmid

#215698

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pY30

Plasmid

#84745

Purpose

Expresses huAsCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast

Plasmid

#167271

Purpose

Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pWF079_LVt-EF1a-TCRGibson_humanTRAC

Plasmid

#233448

Purpose

Gibson assembly of TCRB-P2A-TCRa

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE212.pa

Plasmid

#134930

Purpose

GEF-H1 biosensor that does not have GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

Plasmid

#113889

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE191.pa

Plasmid

#134931

Purpose

GEF-H1 biosensor that has GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-myc

Plasmid

#109422

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope

Tags

BAP, HA, His6, T7, and myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pF420

Plasmid

#123659

Purpose

Produces cofactor F420 in E. coli

Depositor

Colin Jackson

Article

Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

Inserts

CofD
FbiB
FbiD
FbiC

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_R0040

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD1 (full-length, aa 1-481)

Plasmid

#61405

Purpose

Expresses human OTUD1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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011LP-pAAV-TNT4-EmGFP-pA

Plasmid

#120280

Purpose

AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

Depositor

Xander Wehrens

Article

Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

Insert

EmGFP

Use

AAV

Tags

3XFlag and HA

Promoter

TNT Cardiac Troponin T promoter

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 62.4

Plasmid

#123123

Purpose

Human expression vector for deltaproteobacteria CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 62.5

Plasmid

#123124

Purpose

Human expression vector for planctomyces CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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