165,335 results

1. pHR-SFFV_3C-Twin-Strep

   Plasmid

   #113900

   Purpose

   Mammalian protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   SFFV

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP-mCherry-TOSI

   Plasmid

   #172496

   Purpose

   mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   PDCD4 (Pdcd4 Mouse)
   

   Use

   Retroviral

   Tags

   Mcherry

   Expression

   Mammalian

   Promoter

   MLV LTR

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-FOXP2

   Plasmid

   #153146

   Purpose

   Expression of Flag tagged human FOXP2 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXP2 (FOXP2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Pam-FL

   Plasmid

   #42570

   Depositor

   Vijaya Ramesh

   Article

   Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

   Insert

   human MYCBP2 (MYCBP2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Pam-FL clone lacks alternatively spliced exons 52…

   Promoter

   CMV

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCMV-intron myc Rab1a S25N

   Plasmid

   #46777

   Purpose

   expression of S25N Rab1a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab1a S25N mutant (RAB1A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S25N (dominant negative)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pRSET FlipSuc-90u delta1

   Plasmid

   #14475

   Depositor

   Wolf Frommer

   Article

   Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

   Insert

   FlipThuE W283A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   N192D, W283A, 13 amino acids deleted from linkers

   Promoter

   T7

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. araBAD-BCD20-ClpP-BCD17-ClpX

   Plasmid

   #153301

   Purpose

   Expresses clpX and clpP in E.Coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Inserts

   clpX (clpX Escherichia coli)
   clpP (clpP Escherichia coli)
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HERV-Kcon-RT for Split-PE (pJUL3120)

   Plasmid

   #190107

   Purpose

   Expresses HERV-Kcon RT (human codon optimization)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   HERV-Kcon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH001

   Plasmid

   #67641

   Purpose

   High copy Cas9 expression vector with LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   Empty
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-EGFP-C1-HsRCK

    Plasmid

    #25033

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    Rck (DDX6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR223-BLLF1(gp350)

    Plasmid

    #195837

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BLLF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BLLF1 (BLLF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXB-EBNA1

    Plasmid

    #41857

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCM433

    Plasmid

    #15670

    Depositor

    Christopher Marx

    Article

    Marx et al BMC Res Notes. 2008 Feb 26;1:1. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector

    Available Since

    Aug. 31, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

    Plasmid

    #229770

    Purpose

    Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
    

    Use

    Luciferase

    Tags

    LgBiT

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid

    #229769

    Purpose

    Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    

    Use

    Luciferase

    Tags

    SmBiT

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUDP082

    Plasmid

    #103875

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMO14

    Plasmid

    #235736

    Purpose

    Expression of human VPS34 complex II in mammalian cells

    Depositor

    Roger Williams

    Article

    Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

    Inserts

    UVRAG (UVRAG Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    

    Tags

    2xStrep-tagII-2xTEV-ZZ

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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