We narrowed to 11,977 results for: pcDNA

1. pcDNA5-FRT-TO-EGFP-AID

   Plasmid

   #80075

   Purpose

   Mammalian expression vector for N-terminal EGFP-AID tagging

   Depositor

   Andrew Holland

   Insert

   EGFP-AID
   

   Use

   Tags

   AID and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Myc-His RTN3A1

   Plasmid

   #31087

   Depositor

   Jimtong Horng

   Article

   Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

   Insert

   reticulon 3 (RTN3 Human)
   

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-DrFLINC-AKAR-Kras

   Plasmid

   #182334

   Purpose

   Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DrFLINC-AKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3 Flag MKK7b1(glu)

   Plasmid

   #14673

   Depositor

   Roger Davis

   Article

   Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

   Insert

   MKK7b1(Glu) (Map2k7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-269Glu Thr-273Glu

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3 Flag MKK3b(Glu)

   Plasmid

   #50449

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Serine 218 to Glutamic Acid, changed Thre…

   Promoter

   CMV

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5/FRT/TO-AT1R-APEX

   Plasmid

   #96847

   Purpose

   For expression of AT1R-APEX in mammalian cells

   Depositor

   Andrew Kruse

   Article

   Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

   Insert

   AT1R-APEX (AGTR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-MTS-CA-c-Src-FLAG

   Plasmid

   #44654

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed Tyr 530 to Phe

   Promoter

   CMV promoter

   Available since

   May 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO-FNTA-FLAG

   Plasmid

   #86858

   Purpose

   To express FNTA with a FLAG tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTA (FNTA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The sequence of the insert is isoform 2, which di…

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 CMV NAPA-A BGH

   Plasmid

   #92277

   Purpose

   FRET probe; extracellular membrane tethered eGFP

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   Neuron-astrocyte proximity assay - Astrocyte
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/GUG-nLuc-3XFLAG

    Plasmid

    #127301

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-HA-Akt1-aa1-149

    Plasmid

    #73410

    Purpose

    Expresses HA tagged aa1-149 of AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 1-149 only of AKT1

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-hRIPK1-Flag-delDD

    Plasmid

    #112494

    Purpose

    Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of DD domain (AA584-671 deleted)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Flag-Tet3 CD Mut

    Plasmid

    #72222

    Purpose

    Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 3
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET3 catalytic domain (cata…

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/TO-myc-BirA*-NES

    Plasmid

    #112714

    Purpose

    For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    HIV Rev nuclear export signal (NES)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Use

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT-ChemoD-NAD

    Plasmid

    #193826

    Purpose

    CMV overexpression of ChemoD-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoD-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ShadowG: T225R, HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. integrin beta6 pcDNA1 neo

    Plasmid

    #13580

    Depositor

    Dean Sheppard

    Article

    Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human NCKX4-AS-long (pcDNA3.1+)

    Plasmid

    #75211

    Purpose

    Mammalian expression of SLC24A4

    Depositor

    Jonathan Lytton

    Article

    Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

    Insert

    SLC24A4 (SLC24A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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