We narrowed to 11,233 results for: SHA

1. pEGFP-N1 Dynamin1 P294A

   Plasmid

   #112102

   Purpose

   Mammalian expression of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P294A

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIG1_UL136_19kDa_EE

   Plasmid

   #74951

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Glu-Glu tag (EYMPME)

   Expression

   Mammalian

   Mutation

   delta 1-127

   Promoter

   CMV, lentiviral LTR

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIG1_UL136_23kDa_Myc

   Plasmid

   #74949

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   HA Tag (YPYDVPDYA)

   Expression

   Mammalian

   Mutation

   delta 1-99 M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3- myc NES-mCE(K294A) NLS

   Plasmid

   #82467

   Purpose

   Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NLS deleted from mouse Capping Enzyme (Rngtt Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of KRKY from 573-657

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSUPER.retro.puro-shLARP7

   Plasmid

   #113537

   Purpose

   Retroviral vector designed to knock down LARP7.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   shLARP7 (LARP7 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-5X-1-MePCE_aa470-689

   Plasmid

   #113544

   Purpose

   Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-5X-1_MePCE_aa432-689

   Plasmid

   #113543

   Purpose

   Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-5X-1_MePCE_aa400-689

   Plasmid

   #113542

   Purpose

   Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-5X-1_MePCE_aa1-400

   Plasmid

   #113541

   Purpose

   Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSN28

    Plasmid

    #100113

    Purpose

    expresses a chimeric cMYC-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-cMYC fused to a 'weak' VP16 domain (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSN27

    Plasmid

    #100112

    Purpose

    expresses a chimeric cMYC-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-cMYC fused to a 'medium' VP16 domain (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSN26

    Plasmid

    #100111

    Purpose

    expresses a chimeric cMYC-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-cMYC fused to a 'strong' VP16 domain (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. csd2_121-308/pET21a(+)

    Plasmid

    #83293

    Purpose

    csd2, construct: 121-308, pET21a(+) no-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. csd2_140-251/pET21a(+)

    Plasmid

    #83294

    Purpose

    csd2, construct:140-251, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-139 and 252-308

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. csd1_54-312/pET21a(+)

    Plasmid

    #83291

    Purpose

    csd1, construct: 54-312, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-53

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. csd2_121-308/pET28b(+)

    Plasmid

    #83292

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. csd1_125-312/pET28b(+)

    Plasmid

    #83290

    Purpose

    csd1, construct: 125-312, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-124

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIG1_UL136_25kDa_Myc

    Plasmid

    #74954

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

    Expression

    Mammalian

    Mutation

    delta 1-77, M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRevLCav2FeS

    Plasmid

    #60808

    Purpose

    to disrupt iron sulfur cluster in yeast Rev3

    Depositor

    Tahir Tahirov, Youri Pavlov

    Article

    Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 647 C-terminal amino acids and 335 bp of…

    Promoter

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. mutpRL-TK 6xUTR

    Plasmid

    #40761

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    6 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

21. mutpRL-TK 2xUTR

    Plasmid

    #40757

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    2 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

22. mutpRL-TK 4xUTR

    Plasmid

    #40759

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    4 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

23. mutpRL-TK 5xUTR

    Plasmid

    #40760

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    5 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

24. mutpRL-TK 1xUTR

    Plasmid

    #40756

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pCMV10/3xflag-Clock L113E

    Plasmid

    #47339

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L113E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pCMV10/3xflag-Clock F122D

    Plasmid

    #47340

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    F122D

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pCDNA3-HA/BmalF423R

    Plasmid

    #47348

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    F423R

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pEBG-hBEN L38&45P

    Plasmid

    #22182

    Depositor

    Ananda Roy

    Article

    Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Changed Leucine 38 to Proline and changed Leucine…

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pEBB-GFP-hBEN L38&45P

    Plasmid

    #22183

    Depositor

    Ananda Roy

    Article

    Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Luecine 38 to Proline and Leucine 45 to P…

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

30. CMV-sgE1TSS-short-3'box

    Plasmid

    #92165

    Purpose

    sgRNA expression vector for Evx1as RNA tethering assay

    Depositor

    Xiaohua Shen

    Article

    Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

    Insert

    Evx1as short isoform
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge