We narrowed to 78,866 results for: ELL

1. AICSDP-47: CTNNB1-mEGFP

   Plasmid

   #109119

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

   Depositor

   Allen Institute for Cell Science

   Insert

   CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Puro-iNgn3

   Plasmid

   #75337

   Purpose

   doxycycline inducible Ngn3 over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   neurogenin 3 (Neurog3 Mouse)
   

   Expression

   Mammalian

   Promoter

   TRE-tight

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-PASS

   Plasmid

   #193970

   Purpose

   Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

   Depositor

   Guangwei Du

   Article

   Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

   Insert

   PA biosenor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECIA2

   Plasmid

   #47032

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Fc(human IgG1), HRV 3C Protease Cleavage site, He…

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-EF1aL-eGFP-W

   Plasmid

   #126686

   Purpose

   Constitutive, ubiquitous GFP expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   eGFP
   

   Use

   Lentiviral

   Promoter

   EF1aL

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-tetO-MCS

   Plasmid

   #84008

   Purpose

   doxycycline-inducible lentiviral vector

   Depositor

   Stefano Piccolo

   Article

   Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRESneo-EGFP-alpha Tubulin

   Plasmid

   #12298

   Depositor

   Patricia Wadsworth

   Article

   Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

   Insert

   EGFP-alpha Tubulin (TUBA1B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC101_TIMER

   Plasmid

   #103057

   Purpose

   low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RabV CVS-N2c(deltaG)-APEX2-Venus

   Plasmid

   #73467

   Purpose

   Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   APEX2-Venus
   

   Use

   Neurotropic virus

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. modRNAc0-Cas9-2A-GFP

    Plasmid

    #170181

    Purpose

    IVT template plasmid for making modified Cas9GFP RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9-2A-GFP
    

    Use

    Ivt template plasmid

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. RFP-PASS

    Plasmid

    #193971

    Purpose

    Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

    Depositor

    Guangwei Du

    Article

    Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

    Insert

    PA biosenor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Promoter

    none

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

    Plasmid

    #85070

    Purpose

    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    PH-PLCD1 (PLCD1 Human)
    

    Tags

    IRES-sYFP2-PH and mScarlet-H

    Expression

    Mammalian

    Mutation

    PLCD aa 1–170

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1-Luciferase

    Plasmid

    #99283

    Purpose

    All-in-one lentivector with tet-inducible luciferase

    Depositor

    Stephen Tapscott

    Article

    Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_GEM4-Halo-FRB

    Plasmid

    #197057

    Purpose

    Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_GEM7-Halo-FRB

    Plasmid

    #197058

    Purpose

    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMAL

    Plasmid

    #161785

    Purpose

    Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Bidirectional minhCMV and SFFV

    Available Since

    Dec. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe-Dvl2-GFP

    Plasmid

    #109148

    Purpose

    Stable Dvl2 expression

    Depositor

    Mariann Bienz

    Article

    Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

    Insert

    Dvl2 (DVL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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