We narrowed to 16,981 results for: tes

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pLenti_Ef1a:PE2-P2A-GFP

Plasmid

#184445

Purpose

Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLG021 (AVAST type 5)

Plasmid

#157899

Purpose

Expresses the AVAST type 5 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 5 (STAND ATPase with N-terminal SIR2)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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TAL-L1-1.3-Ctrl

Plasmid

#102355

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3

Use

T3 in vitro transcription for expression in mamma…

Tags

FLAG and HA

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

Plasmid

#62905

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

Plasmid

#62945

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pICH44022

Plasmid

#50330

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, P19 suppressor of gene silencing (Tomato Bushy Stunt Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mGFP-hNET

Plasmid

#193364

Purpose

expression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

Depositor

Harald Sitte

Article

Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

Insert

human Norepinephrine Transporter (SLC6A2 Human)

Tags

monomeric GFP (mGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIID

Plasmid

#186827

Purpose

Expresses FLAG-CaMKIID in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2d (Camk2d Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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Hook1-EGFP

Plasmid

#171941

Purpose

Expresses Hook1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Hook1

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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p8092 LentiCRISPR v2 sgNT-1

Plasmid

#163315

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgNT-1

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLG017 (AVAST type 1)

Plasmid

#157895

Purpose

Expresses the AVAST type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 1 (STAND ATPase with N-terminal serine protease)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pWPI-E2F3

Plasmid

#114298

Purpose

Constitutive expression of the E2F3 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

E2F3 (E2F3 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGIG_V5-PPP6C

Plasmid

#92013

Purpose

Expresses V5-PPP6C, includes IRES-Emerald

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

PPP6C (PPP6C Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBW400hrpL-gfp

Plasmid

#61437

Purpose

pBW400hrpL-gfp

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

hrpL-rbs30-gfp-ter

Expression

Bacterial

Promoter

HrpL

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16df

Plasmid

#31505

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16d/f specific (daf-16 Nematode)

Use

RNAi

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pLL3.7_hsa-miR-30c

Plasmid

#25797

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-30c

Use

Lentiviral and RNAi

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pCDFDuet-MKK6-EE p38alpha

Plasmid

#82719

Purpose

Expresses constitutively active human MKK6 and murine p38 alpha.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Inserts

MKK6 (MAP2K6 Human)
p38alpha (Mapk14 Mouse)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pGL3-1574/+120

Plasmid

#35539

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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