-
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only
-
pBItetDT12nGFP
Plasmid#80420Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_ELK3
Plasmid#167750PurposeExpresses ELK3 in mammalian cellsDepositorInsertELK3 (ELK3 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-rtTA-zeo
Plasmid#183233PurposeLentiviral vector expressing the rtTa geneDepositorInsertrtTA-T2A-Zeo
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K214R
Plasmid#127132DepositorInsertRPS3 (RPS3 Human)
UseLentiviralTagsExpressionMutationK214RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)
Plasmid#79621PurposeExpresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionMammalianMutationD10A and H840A for nuclease deficient Cas9PromoterpCMVAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_OTmut
Plasmid#185385PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cellsDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-DVC1-Strep-HA
Plasmid#113481PurposeExpression of human DVC1 with C-terminal strep-HA tagDepositorInsertDVC1 (SPRTN Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
CytERM-mAvicFP1
Plasmid#129510PurposeExpresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)DepositorInsertCytERM-mAvicFP1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEX- HcRed -hLC3ΔG
Plasmid#24992DepositorInserta mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
UseTagsHcRedExpressionMammalianMutationdeleted Gly at 121PromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3-HA-RIPK1 K115R
Plasmid#78835PurposeTo overexpress RIPK1 K115R in Mammalian CellsDepositorInsertRIPK1 (RIPK1 Human)
UseTagsHAExpressionMammalianMutationK115RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST_AfrLEA3m (pBS0911)
Plasmid#185285PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAfrLEA3m
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_PvLEA4_repeats_k3_26 (pBS0762)
Plasmid#185244PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertPvLEA4_repeats_k3_26
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4
Plasmid#136611PurposeDox-inducible lentiviral vector expressing mouse Oct4DepositorInsertOct4 (Pou5f1 Mouse)
UseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero7
Plasmid#187926PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero7DepositorInsertHero7 (SERF2 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero45
Plasmid#187931PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero45DepositorInsertHero45 (SERBP1 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro-luc (shRNA)
Plasmid#153962PurposeExpresses a shRNA against firefly luciferaseDepositorInsertshRNA against firefly luciferase (19-bp-long target)
UseLuciferase, RNAi, and RetroviralTagsN/AExpressionMammalianMutationPromoterU6 promoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-mSOG-bFos-IRES-mCherry
Plasmid#136599PurposeEncodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).DepositorInsertN-mSOG-bFos
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159870PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only