We narrowed to 10,863 results for: HA
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Plasmid#153996PurposeExpress eGFP gene along with HA and BASUDepositorInsertE-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
Plasmid#47458PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.DepositorInsertTALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
cpGFP-Adhiron_flex
Plasmid#165044PurposeFlexible linker control for POLArISact expression in mammalian cellsDepositorInsertcpGFP-Adhiron (connected with a flexible linker)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK2 WT
Plasmid#12182DepositorAvailable SinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMSCV neo WT Akt1
Plasmid#14549DepositorAvailable SinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGP18 (CK2alpha' K69M)
Plasmid#27090DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPPC011
Plasmid#171143PurposeExpression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmidDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LYRa11-RBD-8h
Plasmid#161822PurposeMammalian expression plasmid for RBD of bat coronavirus isolate LYRa11DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T324-D522)PromoterCMVAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RsSHC014-RBD-8h
Plasmid#161826PurposeMammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T321-D519)PromoterCMVAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPT-mCherry
Plasmid#190605PurposeObtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherryDepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationEGFP Kozak sequence has been replaced with a stre…PromoterEIF1-shortAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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FH-TET3-HxDmut-pEF
Plasmid#127896PurposeExpresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079ADepositorAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Rs7327-RBD-8h
Plasmid#161823PurposeMammalian expression plasmid for RBD of bat coronavirus isolate Rs7327DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T321-D519)PromoterCMVAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC5-dual-dCas9VP48-sgTetO
Plasmid#48237PurposeDual expression construct expressing both dCas9VP48 and sgTetO from separate promotersDepositorInsertdCas9VP48 and sgTetO
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Rs4084-RBD-8h
Plasmid#161825PurposeMammalian expression plasmid for RBD of bat coronavirus isolate Rs4084DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T321-D519)PromoterCMVAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Rs4231-RBD-8h
Plasmid#161824PurposeMammalian expression plasmid for RBD of bat coronavirus isolate Rs4231DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T320-D518)PromoterCMVAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-S1977P
Plasmid#64876Purposelentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationS1977P, mimicking the chaos1 mutationPromoterEF1alphaAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:AtFLS2
Plasmid#202186PurposeExpress Arabidopsis thaliana FLS2 gene under UBQ10 promoterDepositorInsertFlagellin sensing 2 (FLS2 Mustard Weed)
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 508)
Plasmid#195030PurposeGAL4-Creb5 fusion construct (full length, 508aa)DepositorInsertGAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV E2F1 1-284
Plasmid#24216PurposeMammalian expression of human E2F1 (aa 1-284) with HA tagDepositorAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-KATNA1
Plasmid#180650PurposeLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.DepositorInsertKATNA1 (KATNA1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GGACAGCACUCCCUUGAAAPromoterAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVE-FH-IN
Plasmid#70071Purpose2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cellsDepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
LAP2-beta-actin in modified pEGFP
Plasmid#34839DepositorAvailable SinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSExpressionMutationPromoterEF1a and human U6Available SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myopathy Loop Mutant Nuclear Myosin I YFP
Plasmid#60243PurposeExpresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activityDepositorInsertNuclear Myosin 1 (Myo1c Mouse)
UseTagsEYFP and NLSExpressionMammalianMutationDeleted I339, I340, G343, E344, E345,L346PromoterCMVAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196A
Plasmid#52974Purposeinsect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196A LKB1 phosphorylation defective mutantPromoterHsp70Available SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 AT1AR-TSTS/4A
Plasmid#45634DepositorInsertAT1AR-TSTS/A (Agtr1a Rat)
UseTagsHAExpressionMammalianMutationThr332, Ser335, Thr336, and Ser338 all changed to…PromoterCMVAvailable SinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Myc-TRFcT
Plasmid#44573DepositorUseRetroviralTagsMycExpressionMammalianMutationTRF2 where TRFH domain (aa42-241) has been replac…PromoterAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZJQIBEBT003
Plasmid#140053PurposeThe pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.DepositorInserteMutS-L157C-G233C-CBM-EGFP
UseTagsHis-TagExpressionBacterialMutationL157C, G233CPromoterT7Available SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_ TET1 CXXC only
Plasmid#124084PurposeExpression of CXXC domain only form of TET1DepositorInsertTET1 - CXXC domain (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF1 alphaAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP
Plasmid#154185Purposeexpresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cellsDepositorInsertretroCHMP3
UseTagsHA and T2A-H2B-BFPExpressionMammalianMutationPromoterEF1-alphaAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXIP TGBRI K to R
Plasmid#19150DepositorInsertTransforming growth factor, beta receptor I (TGFBR2 Human)
UseRetroviralTagsHAExpressionMammalianMutationK420R mutationPromoterAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-hTERT-FF2
Plasmid#164910PurposeExpresses hTERT cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsHA tagExpressionMutationPromoterAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PiuA
Plasmid#128957Purposeexpresses Acinetobacter baumannii PiuA in E. coliDepositorInsertTonB-dependent siderophore receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only