We narrowed to 17,686 results for: She

1. Hs.KRAS4b G12A

   Plasmid

   #83142

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12A

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti pTERT sgRNA2(MS2) mCherry

   Plasmid

   #171055

   Purpose

   Targeting of Vp64-dCas9 activation system to hTERT promoter

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   pTERT sgRNA2(MS2) mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ANKRA2

   Plasmid

   #36904

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ANKRA2 (ANKRA2 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   amino acid residues 148-313 (ANK repeats)

   Available Since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pPB-CAG-cNanog-pgk-hph

   Plasmid

   #74907

   Purpose

   Mammalian expression of cNanog

   Depositor

   Jose Silva

   Article

   Theunissen et al Development. 2011 Nov;138(22):4853-65. d

   Insert

   Nanog
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MS2_SC2_S_Lambda

   Plasmid

   #175048

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Lambda SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   Lambda (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pMT-DREDD-V5-His

   Plasmid

   #63749

   Purpose

   Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
   

   Tags

   V5-6XHis

   Expression

   Insect

   Promoter

   pMT

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. POT4-TDH3p-ycrtI-ADH1t

   Plasmid

   #65355

   Purpose

   TU for combinatorial construction of ?-carotene pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   TDH3p-ycrtI-ADH1t
   

   Use

   Unspecified

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTRIPZ (M)-HT-Cbx7-ATLm

   Plasmid

   #82522

   Purpose

   Lentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 7 (CBX7 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   mutation of RKR70-72, K75, and KR77-78 to AGA70-7…

   Promoter

   CMV

   Available Since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK371

   Plasmid

   #73596

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-12 fatty acid desaturase (form 3)
   

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Point mutants in FAD2-3 ORF relative to deposited…

   Promoter

   GAL1 and GAL10

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-rbNHERF1

    Plasmid

    #31636

    Depositor

    Edward Weinman

    Article

    Voltz et al J Biol Chem. 2007 Nov 16. 282(46):33879-

    Insert

    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Sox17 Flox

    Plasmid

    #23335

    Depositor

    Sean Morrison

    Article

    Kim et al Cell. 2007 Aug 10. 130(3):470-83.

    Insert

    Sex determining region Y-box containing gene 17 (Sox17 Mouse)
    

    Use

    Mouse Targeting

    Mutation

    None

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pUASp YFP Rho1 DN

    Plasmid

    #52231

    Purpose

    Drosophila expression of YFP-Rho1 dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rho1 (Rho1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T19N; DN mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGEX2TK-YAP-WW1

    Plasmid

    #104305

    Purpose

    bacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylated

    Depositor

    Marius Sudol

    Insert

    YAP WW1 domain (YAP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    WW domain 1

    Promoter

    tac

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MS2_SC2_S_Alpha

    Plasmid

    #175045

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Alpha (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. MS2_SC2_S_Beta

    Plasmid

    #175046

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Beta (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid

    #159913

    Purpose

    Mutagenesis of Kcnj6

    Depositor

    Larry Zweifel

    Insert

    Kcnj6 (Kcnj6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. cisRAJ31-GFP-LAA

    Plasmid

    #69928

    Purpose

    Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    PllacO1-cisRAJ31-GFP-LAA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PlLacO1

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. circRAJ31-Toehold_mutant

    Plasmid

    #69930

    Purpose

    Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    plTet-circRAJ31_toeholdmutant
    

    Use

    Synthetic Biology

    Mutation

    4 bases mutated in toehold of riboregulator

    Promoter

    PltetO1-2

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 4IA9

    Plasmid

    #51320

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. HECW2

    Plasmid

    #32875

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECW2 (HECW2 Human)
    

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

21. CMV-Y-GECO2m

    Plasmid

    #105070

    Purpose

    A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2m
    

    Tags

    6x His tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S

    Plasmid

    #40394

    Depositor

    Michael J Fox Foundation MJFF

    Inserts

    SUMO
    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    6xHis and HisTEV

    Mutation

    aa1867-2161,C2024S/C2025S

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. 4PZI

    Plasmid

    #61955

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLL4:JMC054-D08:C226821 (KMT2B Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

24. Hs.KRAS4b C185S

    Plasmid

    #83139

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), C185S mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    C185S

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PIWIL2

    Plasmid

    #32042

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIWIL2 (PIWIL2 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    PAZ domain only (amino acids 386-533)

    Available Since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

26. EPB41

    Plasmid

    #32040

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPB41 (EPB41 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    FERM domain only (amino acids 1-279)

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

27. HSD17B8

    Plasmid

    #38853

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B8 (Hsd17b8 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pDM_noro_A1_lacZA

    Plasmid

    #118818

    Purpose

    T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A1) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch A1
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GHRHR

    Plasmid

    #39173

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GHRHR (GHRHR Human)
    

    Use

    Baculovirus expression

    Tags

    Signal-His6-TEV

    Mutation

    N20->Q mutation of putative glycosylsation sit…

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. Gli3ZF in AINGpCAGGS

    Plasmid

    #121972

    Purpose

    Gli3 zinc fingers

    Depositor

    James Briscoe

    Insert

    Gli3ZF (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus and C terminus deletion only nt 1411-1…

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. POT5-CYC1p-ycrtYB-ADH1t

    Plasmid

    #65360

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    CYC1p-ycrtYB-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Hs.KRAS4b Q61K

    Plasmid

    #83164

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61K

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTorPE-Y-GECO2.2s

    Plasmid

    #105069

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2.2s
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAdTrack-mLIP

    Plasmid

    #49197

    Purpose

    adenoviral shuttle vector with mouse C/EBPbeta LIP fragment

    Depositor

    Jed Friedman

    Insert

    C/EBPbeta (LIP fragment) (Cebpb Mouse)
    

    Use

    Adenoviral

    Mutation

    only the LIP fragment

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pS2-FLAG-Sestrin2-E451A

    Plasmid

    #73679

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    Sesn2

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

36. Myc-gamma2s-EGFP

    Plasmid

    #118040

    Purpose

    GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG2 (Gabrg2 Rat)
    

    Tags

    EGFP and cMyc epitope EQKLISEEDL inserted between…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. OA959C

    Plasmid

    #104968

    Purpose

    Expresses anti-ZIKV transgene in Ae. aegypti

    Depositor

    Omar Akbari

    Article

    Buchman et al Proc Natl Acad Sci U S A. 2019 Feb 5. pi

    Insert

    Anti-Zika Transgene
    

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-CW3SL-Myr-mCherry-Dr-TrkA

    Plasmid

    #127962

    Purpose

    Myr mCherry DrTrkA expression under CAMK promoter

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

    Insert

    mCherry DrTrkA
    

    Use

    AAV

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid

    #110378

    Purpose

    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    crystallin zeta (CRYZ Human)
    

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDNR-hSox2

    Plasmid

    #49389

    Purpose

    donor vector for cloning human Sox2 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    none

    Available Since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only