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pAAV-anti-GFP/BFP(LaG2) PAGER(FL)

Plasmid

#230022

Purpose

Expresses anti-GFP/BFP PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(Gq)

Plasmid

#230006

Purpose

Expresses anti-GFP PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-VEGF(Nb35) PAGER(FL)

Plasmid

#230025

Purpose

Expresses anti-VEGF PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-Nb35-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(G12)

Plasmid

#230009

Purpose

Expresses anti-GFP PAGER(G12) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM1Dx12

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NmCherry-mAID

Plasmid

#140603

Purpose

Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd9

Plasmid

#42261

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 9 (Fzd9 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)

Plasmid

#191139

Purpose

Expression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gates

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t17d9b

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-225

Plasmid

#21306

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pAC-crRNA-Km

Plasmid

#158712

Purpose

Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

AcGFP (for crRNA cloning)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

TetO

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Blue opsin mcherry ---- pcDNA3.1

Plasmid

#62932

Purpose

Blue opsin tagged with mcherry on the C terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

blue opsin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC1_dual-MTS-3x-Set I

Plasmid

#162763

Purpose

Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3

Expression

Mammalian

Promoter

U6

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shACO1

Plasmid

#169884

Purpose

Suppress ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

shRNA against ACO1

Use

Lentiviral and RNAi

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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PXWABS62(E11-TEV)

Plasmid

#123157

Purpose

For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

ParsR-ABS62, Pecf11

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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GLUT9-eGFP/pcDNA-DEST47

Plasmid

#18730

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
MAC_C_SCO1

Plasmid

#111675

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

SCO1 (SCO1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAID1.2-CMV-NmCherry-mAID

Plasmid

#140601

Purpose

Expression of OsTIR1 and mCherry-mAID protein in DT40 cells under the control of CMV promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGUS (GB0208)

Plasmid

#68203

Purpose

Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

GUS coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd6

Plasmid

#42258

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 6 (Fzd6 Mouse)

Expression

Mammalian

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only