We narrowed to 12,197 results for: SHA;

Showing: 4521 - 4540 of 12197 results

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sReach-mTurquoise2

Plasmid

#98840

Purpose

Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

sReach-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mNeon

Plasmid

#207687

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Puro

Plasmid

#227262

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
mTagBFP2-Endosomes-14

Plasmid

#55293

Purpose

Localization: Endosomes, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Endosomes (RHOB Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
p15A_SpyCas9_CmR

Plasmid

#119162

Purpose

Expresses S. pyogenes Cas9

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

Cas9 from S. pyogenes (NEWENTRY )

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-LKB1-KM

Plasmid

#21148

Depositor

Junying Yuan

Article

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1-KM (Stk11 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

K78M

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
qTAG-N-Puro-3xFLAG

Plasmid

#207695

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-Wnt5A-V5

Plasmid

#35930

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPP444

Plasmid

#158802

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 77L

Plasmid

#19308

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Mutation

S77L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3A-V5

Plasmid

#35927

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

April 20, 2012

Availability

Academic Institutions and Nonprofits only

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mRuby2-pBAD

Plasmid

#54771

Purpose

Localization: Protein Expression Vector , Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and mRuby2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-3xFLAG-Zeo

Plasmid

#207739

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-miniTurbo-Puro

Plasmid

#207725

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Sfp R4-4 mutant

Plasmid

#173491

Purpose

Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

Depositor

Jun Yin

Article

Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

Insert

Sfp-R44

Tags

His tag

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST26-N-FLAG

Plasmid

#79549

Purpose

Destination vector containing an N-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits
pACS131

Plasmid

#204596

Purpose

Histone H3.1K27M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 K27M (H3C1 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mKOκ-EC18154

Plasmid

#137077

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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