We narrowed to 40,052 results for: lat

1. Tet-O-FUW-EGR3-P2A-mCherry

   Plasmid

   #203861

   Purpose

   Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   EGR3 (EGR3 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

   Plasmid

   #172611

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDKN1B (CDKN1B Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-MKK1

   Plasmid

   #107973

   Purpose

   expression of MAP Kinase Kinase

   Depositor

   Edwin Krebs

   Article

   Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

   Insert

   MKK1 (MAP2K1 Human)
   

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   CMV

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRESh-GFPII-MB-Dlk1

   Plasmid

   #108930

   Purpose

   GFP-tagged vector expressing membrane-bound Dlk1

   Depositor

   Anne Ferguson-Smith

   Article

   Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

   Insert

   Dlk1 (Dlk1 Mouse)
   

   Tags

   GFPII

   Expression

   Mammalian

   Mutation

   lacks coding sequence for the juxtamembrane motif…

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIZ GFP PODXL delta PBM + Ezrin

   Plasmid

   #202418

   Purpose

   Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   Ezrin and GFP

   Mutation

   delete PDZ binding motif

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-MKK1K97A

   Plasmid

   #107974

   Purpose

   expression of a dominant negative MAP Kinase Kinase

   Depositor

   Edwin Krebs

   Article

   Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

   Insert

   MKK1 K97A (MAP2K1 Human)
   

   Expression

   Mammalian

   Mutation

   dominant negative; see Depositor Comments below

   Promoter

   CMV

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF-DUET1-DHDDS/Sumo-NgBR

   Plasmid

   #203162

   Purpose

   Expression of human cis-prenyltransferase complex for purification

   Depositor

   William Sessa

   Article

   Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

   Inserts

   DHDDS (DHDDS Human)
   NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)
   

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   truncation

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-Htt171-18Q-myc-WPRE

   Plasmid

   #107936

   Purpose

   Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

   Depositor

   Johan Jakobsson

   Article

   Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

   Insert

   Exon 1 of wild type HTT (HTT Human)
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

   Plasmid

   #71828

   Purpose

   Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   U6

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SARS CoV-2 HexaPro spike (bio-His)

    Plasmid

    #166856

    Purpose

    Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.

    Depositor

    Evelien Bunnik

    Article

    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

    Insert

    SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
    

    Tags

    8xHis and Biotinylation site

    Expression

    Mammalian

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-Dnmt3a3L-sc27

    Plasmid

    #71827

    Purpose

    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

    Depositor

    Albert Jeltsch

    Article

    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

    Insert

    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRESh-GFPII-S-Dlk1

    Plasmid

    #108931

    Purpose

    GFP-tagged vector expressing secreted isoform of Dlk1

    Depositor

    Anne Ferguson-Smith

    Article

    Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

    Insert

    Dlk1 (Dlk1 Mouse)
    

    Tags

    GFPII

    Expression

    Mammalian

    Mutation

    contains sequences from full length Dlk1A up to t…

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR207 SARS-CoV-2 NSP1 Delta RC

    Plasmid

    #164522

    Purpose

    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Insert

    NSP1 Delta RC (ORF1ab )
    

    Expression

    Mammalian

    Mutation

    Insert of NSP1 gene's CDS from SARS-CoV-2 is…

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-mFgf21pro2-luc

    Plasmid

    #101798

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-BRAF-REG-Halo

    Plasmid

    #194771

    Purpose

    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-mFgf21pro3-luc

    Plasmid

    #101799

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE-Tau-cYFP

    Plasmid

    #92205

    Purpose

    Retroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Retroviral

    Tags

    YFP(156-239)

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only