We narrowed to 14,039 results for: TIM

1. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

   Plasmid

   #174642

   Purpose

   Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)
   

   Tags

   SSPB(micro)-Venus

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

   Plasmid

   #133788

   Purpose

   CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized St3Cas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PIP5K1A gRNA (BRDN0001487080)

   Plasmid

   #77962

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1A (PIP5K1A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PIP5K1B gRNA (BRDN0001146307)

   Plasmid

   #77085

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1B (PIP5K1B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PIP5K1B gRNA (BRDN0001148929)

   Plasmid

   #77086

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1B (PIP5K1B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. non-targeting control gRNA (BRDN0001145885)

   Plasmid

   #80196

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCSF-R(m40)-luc

   Plasmid

   #12425

   Depositor

   Dong-Er Zhang

   Article

   Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

   Insert

   M-CSF receptor promoter fragment (CSF1R Human)
   

   Use

   Luciferase

   Mutation

   PU.1 binding site mutation (created a KpnI site i…

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

8. PRPS2 gRNA (BRDN0001147559)

   Plasmid

   #78005

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRPS2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRPS2 (PRPS2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRPS2 gRNA (BRDN0001487102)

   Plasmid

   #78006

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRPS2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRPS2 (PRPS2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ADCK3 gRNA (BRDN0001147832)

    Plasmid

    #77321

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ADCK3 gRNA (BRDN0001146491)

    Plasmid

    #77323

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pONL-C1_Rab11a

    Plasmid

    #65707

    Purpose

    Expresses ONL-Rab11a in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Rab11a (Rab11a Mouse)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2-7xTcf-NLS-ONL-CP

    Plasmid

    #65716

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. ADRBK2 gRNA (BRDN0001487158)

    Plasmid

    #77943

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADRBK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADRBK2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-A0LWB3

    Plasmid

    #103070

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRTTO-NHASt-codoptUL50

    Plasmid

    #135101

    Purpose

    Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL50 codon optimized
    

    Tags

    HA-Strep-Strep

    Expression

    Mammalian

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2-7xTcf-NLS-YNL-CP

    Plasmid

    #65714

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRIM27 gRNA (BRDN0001149189)

    Plasmid

    #77514

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM27

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM27 (TRIM27 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRIM27 gRNA (BRDN0001149307)

    Plasmid

    #77515

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM27

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM27 (TRIM27 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. HEL binding scFv D44.1

    Plasmid

    #111718

    Purpose

    Yeast Surface Display of D44.1 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    D44.1
    

    Tags

    cmyc tag

    Expression

    Yeast

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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