We narrowed to 13,291 results for: cDNA

1. pcDNA3-MTS-CA-c-Src-FLAG

   Plasmid

   #44654

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed Tyr 530 to Phe

   Promoter

   CMV promoter

   Available Since

   May 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT TO GFP11-LRRK2_G2019S

   Plasmid

   #231175

   Purpose

   Expresses GFP-11-LRRK2 G2019S in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   GFP11-LRRK2 (G2019S) (LRRK2 Human)
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   G2019S

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-AT1R-APEX

   Plasmid

   #96847

   Purpose

   For expression of AT1R-APEX in mammalian cells

   Depositor

   Andrew Kruse

   Article

   Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

   Insert

   AT1R-APEX (AGTR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Yellow Cameleon-Nano50/pcDNA3

   Plasmid

   #51964

   Purpose

   mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano50
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-sACt-mCherry-NES

   Plasmid

   #138215

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NES (Adcy10 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)/CUG-nLuc-3XFLAG

   Plasmid

   #127300

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-H2A-Akt-STOPS

   Plasmid

   #175010

   Purpose

   Akt-STOPS targeted to the nucleus via histone H2A.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   H2A-mCherry-Akt-STOPS
   

   Use

   Tags

   Histone H2A and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-HA-Akt1-aa1-149

   Plasmid

   #73410

   Purpose

   Expresses HA tagged aa1-149 of AKT1

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Expresses aa 1-149 only of AKT1

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3 Flag MKK7b1(glu)

   Plasmid

   #14673

   Depositor

   Roger Davis

   Article

   Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

   Insert

   MKK7b1(Glu) (Map2k7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-269Glu Thr-273Glu

   Promoter

   Available Since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Use

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Flag-Tet3 CD Mut

    Plasmid

    #72222

    Purpose

    Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 3
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET3 catalytic domain (cata…

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

12. integrin beta6 pcDNA1 neo

    Plasmid

    #13580

    Depositor

    Dean Sheppard

    Article

    Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-N-mEGFP-PARP6

    Plasmid

    #178005

    Purpose

    Expresses N-terminally mEGFP-tagged PARP6

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP6 (PARP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT/TO-N-mEGFP-PARP15

    Plasmid

    #178000

    Purpose

    Expresses N-terminally mEGFP-tagged PARP15

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/TO-N-mEGFP-PARP11

    Plasmid

    #177998

    Purpose

    Expresses N-terminally mEGFP-tagged PARP11

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP11 (PARP11 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

    Plasmid

    #115789

    Purpose

    Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    NDP (NDP Human)
    

    Use

    Tags

    alkaline phosphatase and flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid

    #182334

    Purpose

    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DrFLINC-AKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-N-Myc_DED/AAA USP7

    Plasmid

    #131253

    Purpose

    Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert USP7 aspart…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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