161,256 results

1. ePE6d

   Plasmid

   #229029

   Purpose

   Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

   Depositor

   Li Juan

   Article

   Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PD-L1-SNAP

   Plasmid

   #223577

   Purpose

   Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-MerCreMer-hygro

   Plasmid

   #188982

   Purpose

   Retroviral expression of tamoxifen-inducible Cre recombinase

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   Tamoxifen-inducible Cre recombinase
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a HAT epitope

   Plasmid

   #129445

   Purpose

   expression of HAT epitope

   Depositor

   Oskar Laur

   Insert

   HAT epitope
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-EWSR1 c1655t No Stop

   Plasmid

   #84892

   Purpose

   DONOR vector for Gateway cloning of EWSR1 c1655t No Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Gateway donor

   Tags

   Expression

   Mutation

   c1655t No Stop

   Promoter

   none

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pDONR221-EWSR1 g1532c No Stop

   Plasmid

   #84890

   Purpose

   DONOR vector for Gateway cloning of EWSR1 g1532c No Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Gateway donor

   Tags

   Expression

   Mutation

   g1532c No Stop

   Promoter

   none

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pME-mVenus-CAAX

   Plasmid

   #75153

   Purpose

   Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mVenus-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYD1

   Plasmid

   #73447

   Purpose

   expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

   Depositor

   Dane Wittrup

   Article

   Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

   Type

   Empty backbone

   Use

   Tags

   6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-Ii_VSVG-SBP-EGFP

   Plasmid

   #65300

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-TLR10-YFP

    Plasmid

    #13643

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 10 (TLR10 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid

    #185916

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. JD690

    Plasmid

    #160397

    Purpose

    Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::citrus GRF4-GIF1 chimera
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti hSyn PKM1

    Plasmid

    #220920

    Purpose

    Neuronal specific expression of PKM1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PKM1 (PKM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-GFP1-10-pDEST-N

    Plasmid

    #118368

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CKIIa-stGtACR2-FusionRed

    Plasmid

    #105669

    Purpose

    Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde and AAV1

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG R2pH-LAMP1-3xFLAG

    Plasmid

    #157940

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Chick beta actin pomoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-Ef1a-Coff/Fon-mCherry

    Plasmid

    #137134

    Purpose

    Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCXLE-mCherry

    Plasmid

    #193293

    Purpose

    episomal vector expressing mCherry fluorescent protein

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSBbi 41BBL-CD86

    Plasmid

    #183244

    Purpose

    Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    CD86, 41BBL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAcGP67A - murine TfR

    Plasmid

    #12392

    Depositor

    Pamela Bjorkman

    Insert

    transferrin receptor (Tfrc Mouse)
    

    Use

    Tags

    6X-His tag, Factor Xa cleavage site, and leader p…

    Expression

    Insect

    Mutation

    See comments section

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLIC-SM-HA

    Plasmid

    #111188

    Purpose

    High affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)

    Depositor

    Silvia Moreno

    Article

    Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

    Type

    Empty backbone

    Use

    Toxoplasma gondii expression

    Tags

    Spaghetti Monster HA Tag

    Expression

    Mutation

    Promoter

    Endogenous

    Available since

    Aug. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. PB-CMV-MCS-Puro

    Plasmid

    #219794

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCX459

    Plasmid

    #229214

    Purpose

    CRISPR array plasmid for MAP1B (Spacers 1-24)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for MAP1B (Spacers 1-24)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLenti CMV Blast empty (w263-1)

    Plasmid

    #17486

    Purpose

    3rd gen lentiviral empty control vector, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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