We narrowed to 9,400 results for: Coli
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Plasmid#186425Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoterDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-NEUR
Plasmid#53271PurposeContains the Erwinia herbicola (Pantoea agglomerans) Eho10 genes crtE and crtB, and the Rhodobacter capsulatus crtI gene and thereby produces neurosporene in Escherichia coliDepositorInsertcrtI
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodA
Plasmid#89612PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase A
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
UseTagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GPromoterAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
UseTagsExpressionBacterialMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD117A
Plasmid#218141PurposeExpresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coliDepositorInsertSD40
UseTagsAviTag-6xHis-MBP-TEV-(GGGGS)2ExpressionMutationPromoterAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)
Plasmid#111253PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptideDepositorInsertTorA signal peptide
UseTagsExpressionBacterialMutationPromoterPcAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlh_N + pSiMPlh_C
Plasmid#134316PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInserthygR_gp41-1 N-intein + hygR_gp41-1 C-intein
UseTagsExpressionBacterialMutationP254Q in hygR_gp41-1 N-inteinPromoterAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-C
Plasmid#190966PurposeTest the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1-CTD
Plasmid#136649PurposeExpression of SH3BP1-CTD-GST fusion protein in E. coliDepositorInsertSH3BP1-CTD (SH3BP1 Human)
UseTagsExpressionBacterialMutationContains only C-terminal domainPromoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1-NTD
Plasmid#136648PurposeExpression of SH3BP1-NTD-GST fusion protein in E. coliDepositorInsertSH3BP1-NTD (SH3BP1 Human)
UseTagsExpressionBacterialMutationContains only N-terminal domainPromoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS1034
Plasmid#63796PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection markerDepositorInsertCBP-3xFLAG-TEV-Protein A followed by KlLEU2
UseE. coli cloning vectorTagsCBP-3xFLAG-TEV-Protein AExpressionMutationPromoternoneAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Affibody EGFR:1907.BirA*-pET301/NT-DEST
Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
UseTagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen1
Plasmid#78180PurposeExpresses rsGreen1 in E. coli strains.DepositorInsertrsGreen1
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEA02
Plasmid#172193PurposeSuicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattL
UseTagsExpressionBacterialMutationPromoterAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
1BR9-mCherry
Plasmid#193864PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.DepositorInsertmCherry
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1
Plasmid#98150PurposeExpresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1
UseTagsHis6-TEVExpressionBacterialMutationWTPromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SOMA
Plasmid#118935PurposeUsed to express the SOMA FRET sensor in E. coli.DepositorInsertSOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
UseTagsExpressionBacterialMutationPromoterAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPYngK-hp
Plasmid#48118PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertsignal peptide of YngK
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a - CPX
Plasmid#89620Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertCytosolic Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS
Plasmid#127365PurposeBinary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
UseTagsGS linker, NLS, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A-R69A
Plasmid#127787PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32, Arg36 and Arg69 to AlaPromoterAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1-Pct5.1-crRNA(dadR)
Plasmid#191634PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)DepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodB
Plasmid#89613PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase B
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T5-ASG_ClpXP
Plasmid#153299PurposeExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917DepositorInsertASG
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only