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pTOMM20-Rhobin2-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239022

Purpose

Co-overexpression of TOMM20-Rhobin2 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

TOMM20-Rhobin2 (TOMM20 Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTOMM20-Rhobin9-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239023

Purpose

Co-overexpression of TOMM20-Rhobin9 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

TOMM20-Rhobin9 (TOMM20 Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin9-SEC61B-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239025

Purpose

Co-overexpression of Rhobin9-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin9-SEC61B (SEC61B Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG CEP68

Plasmid

#236463

Purpose

transient overexpression of Cep68 in mammalian cells

Depositor

Michele Pagano

Article

Pagan et al Nat Cell Biol. 2015 Jan;17(1):31-43. doi

Insert

Cep68 (CEP68 Human)

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG FBXO15

Plasmid

#236456

Purpose

transient overexpression of FBXO15 in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

FBXO15 (FBXO15 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235326

Purpose

PiggyBac integration vector for ComMAND open-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235318

Purpose

Lentiviral expression of mRuby2 with 3' microRNA only

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235327

Purpose

PiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235268

Purpose

Lentiviral expression of ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235317

Purpose

Lentiviral expression of mRuby2 with intronic microRNA only

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262V

Plasmid

#234607

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262N

Plasmid

#234608

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3G1S+4V

Plasmid

#234612

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to Asn

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4GSV (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

G204V, S231V, G274V, G303V

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAB17 (punc-17::QuasAr)

Plasmid

#214888

Purpose

Expresses the voltage sensor QuasAr2 in cholinergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

QuasAr2

Expression

Worm

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED solvvol

Plasmid

#232958

Purpose

Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD 5W D262V

Plasmid

#234617

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_5W D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

F222W, F228W, Y237W, D262V, Y305W, F320W

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

Plasmid

#200283

Purpose

Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)

Use

Lentiviral

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipT-2A-IvfChr (citrine, KV2.1)

Plasmid

#221619

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1

Use

AAV

Mutation

ZipACR (I151T) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipT-2A-IvfChr (mCherry, KV2.1)

Plasmid

#221621

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-mCHerry with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-mCherry-Kv2.1

Use

AAV

Mutation

ZipACR (I151T) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1)

Plasmid

#221618

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151V mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1

Use

AAV

Mutation

ZipACR (I151V) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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