We narrowed to 10,133 results for: ada

1. pcDNA SF-HERC2 F2

   Plasmid

   #55785

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

2. LentiCRISPR v2-sgAMPK alpha1 clone1

   Plasmid

   #162119

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. xCas9(3.6)-ABE(7.10)

   Plasmid

   #108387

   Purpose

   Mammalian expression vector for xCas9(3.6)-ABE

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-ABE(7.10)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Fireworks GFP[PTC-] (AVA2598)

   Plasmid

   #85445

   Purpose

   Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Expression

   Mammalian

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 5HT6-YC3.60

   Plasmid

   #48339

   Purpose

   FRET reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
   

   Tags

   YC3.60

   Expression

   Mammalian

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

6. MAC_N_IRF8

   Plasmid

   #167763

   Purpose

   Expresses IRF8 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   IRF8 (IRF8 Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-FKBP-GFP-NAP1

   Plasmid

   #208862

   Purpose

   Stably express GFP-tagged NAP1 for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191156

   Purpose

   Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:GAPDH.s1:EGFP-3xMS2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEX-CFP-hLC3WT

   Plasmid

   #24985

   Depositor

   Isei Tanida

   Article

   Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

   Insert

   a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Fireworks RFP[PTC-] (AVA2515)

    Plasmid

    #85443

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid

    #213965

    Purpose

    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP.N1-HDAC6.delta Buz

    Plasmid

    #36190

    Depositor

    Tso-Pang Yao

    Article

    Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

    Insert

    HDAC6..delta Buz (HDAC6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal ub binding domain (BUZ…

    Promoter

    CMV

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TS MS-Sensor

    Plasmid

    #199788

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TS MS-Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

    Plasmid

    #222917

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe Angptl4 puro

    Plasmid

    #19156

    Depositor

    Joan Massague

    Article

    Padua et al Cell. 2008 Apr 4. 133(1):66-77.

    Insert

    angiopoietin-like 4 (ANGPTL4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

16. Circular 100,50 (RAB7A)

    Plasmid

    #170117

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 100,50 guide RNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA Flag Yap1 Y357F

    Plasmid

    #18882

    Depositor

    Yosef Shaul

    Article

    Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

    Insert

    Yap1 Y357F (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y357F

    Available Since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 4LNDDE488Q-pCDNA3.1(+)

    Plasmid

    #172228

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    changed the Glutamic acid 488 in the Deaminase Do…

    Promoter

    CMV

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRXIP Lamp1-Venus

    Plasmid

    #89937

    Purpose

    Expresses Lamp1 tagged with Venus in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    rat Lamp1 (Lamp1 Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge