We narrowed to 9,131 results for: ef1;
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Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_816
Plasmid#216098PurposeCRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)DepositorInsertCas12a [EnAs]
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one_Bip gRNA1
Plasmid#183696PurposeExpresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selectiveDepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_R1683X
Plasmid#198111Purposelentiviral expression of hCOL4A4_R1683Xin mammalian cellsDepositorAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_C1684X
Plasmid#198112Purposelentiviral expression of hCOL4A4_C1684X in mammalian cellsDepositorAvailable SinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_C29X
Plasmid#198107Purposelentiviral expression of hCOL4A5_C29X in mammalian cellsDepositorAvailable SinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_R1563X
Plasmid#198109Purposelentiviral expression of hCOL4A4_R1563Xin mammalian cellsDepositorAvailable SinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc_S36X
Plasmid#198108Purposelentiviral expression of hCOL4A4_S36X in mammalian cellsDepositorAvailable SinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_CI-hCOL4A5-Nluc
Plasmid#198106Purposelentiviral expression of hCOL4A4 in mammalian cellsDepositorAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-Rabex-5
Plasmid#197043PurposeExpression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertRabex-5 (RABGEF1 Bovine)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e VPR
Plasmid#188516PurposeExpresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)DepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-VPRExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2604
Plasmid#175088PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationS644AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2602
Plasmid#175086PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationT424A, T438AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2603
Plasmid#175087PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationT504A, T508AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem209
Plasmid#175154PurposeLentiviral expression of V5-tagged mouse Tmem209DepositorAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Zdhhc6-V5
Plasmid#175146PurposeLentiviral expression of mouse Zdhhc6-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Ptdss2-V5
Plasmid#175143PurposeLentiviral expression of mouse Ptdss2-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-mRnf185
Plasmid#175126PurposeLentiviral expression of V5-tagged mouse Rnf185DepositorAvailable SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem43-V5
Plasmid#175111PurposeLentiviral expression of mouse Tmem43-V5DepositorAvailable SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
L2_NOP1gRNA-Cas9-CsA
Plasmid#136139PurposePlasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygRDepositorInsertp5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only