We narrowed to 5,331 results for: pcDNA3.1

1. pcDNA3-HLA-cMyc-EcopT1R1

   Plasmid

   #113962

   Purpose

   mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R1 (TAS1R1 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   truncate N-terminal 24 residues

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNAZeo(-) LAMP2C

   Plasmid

   #89342

   Purpose

   Expresses human LAMP-2C in mammalian cells

   Depositor

   Janice Blum

   Article

   Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

   Insert

   Lysosomal-associated membrane protein 2C (LAMP2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-MED22

   Plasmid

   #15423

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED22 (MED22 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. Arrb1-RFP

   Plasmid

   #35401

   Depositor

   Robert Lefkowitz

   Article

   Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

   Insert

   beta-arrestin-1 (Arrb1 Rat)
   

   Tags

   RFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3 proHB-EGF CS

   Plasmid

   #11601

   Depositor

   Rosalyn Adam

   Article

   Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

   Insert

   HB-EGF (HBEGF Human)
   

   Tags

   His and myc

   Expression

   Mammalian

   Mutation

   Constitutively secreted HB-EGF: C-terminal amino …

   Available Since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-myc-his-BACH1 K52R

   Plasmid

   #17643

   Depositor

   Ronny Drapkin

   Article

   Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

   Insert

   BACH1 (BRIP1 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   The lysine residue at position 52 was changed to …

   Available Since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-HA-DNM2-R465W

   Plasmid

   #155262

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Arginine 465 to Tryptophan

   Available Since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-mito-CAR-GECO1

   Plasmid

   #46022

   Purpose

   Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   CAR-GECO1
   

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

9. FLAG-MED18

   Plasmid

   #15419

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED18 (MED18 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-sACE2(WT)

    Plasmid

    #145147

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Opto-M1R

    Plasmid

    #106069

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M1R (CHRM1 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. hHSF1 S303/7A

    Plasmid

    #118365

    Purpose

    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A and S307A

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Expression

    Insect

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY31

    Plasmid

    #84746

    Purpose

    Expresses huLbCpf1-P2A-puro and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. HiBiT-FZD6

    Plasmid

    #195844

    Purpose

    Expresses human FZD6 with an N-terminal HiBiT; contains M345L

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD6 (FZD6 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    M345L

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. hHSF1 RNAi resistant

    Plasmid

    #118347

    Purpose

    This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    RNAi resistance mutations (does not change amino …

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Opto-GPR146

    Plasmid

    #106047

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR146 (GPR146 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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