We narrowed to 5,444 results for: 129

1. pAAV-hSyn-GFP-IRES-hSNCA (S129A)

   Plasmid

   #185718

   Purpose

   AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Changed Ser 129 to Ala

   Promoter

   hSyn

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHBS1292 TDP43 RRM-GFP G368W+W385G

   Plasmid

   #107838

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G368W and W385G mutations in LLPS reporter

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEST-p15A-J23151-deGFP-T500 (129p)

   Plasmid

   #70205

   Purpose

   Promoter panel

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

   Plasmid

   #210604

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional activator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   hyperdCas12a-miniVPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

   Plasmid

   #210605

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional base editor

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   ABE8e-hyperdCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A

   Plasmid

   #32456

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S129A, S133A, S137A (S3V, G96R, and P108L also pr…

   Promoter

   SV40

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D

   Plasmid

   #32457

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S129D, S133D, S137D (S3V, G96R, and P108L also pr…

   Promoter

   SV40

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

   Plasmid

   #229823

   Purpose

   A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AiP20129 - pAAV-AiE3288m-minBG-SYFP2-WPRE3-BGHpA

   Plasmid

   #230563

   Purpose

   AiE3288m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AiP1358 - pAAV-AiE2129m-minBG-SYFP2-WPRE3-BGHpA

    Plasmid

    #230530

    Purpose

    AiE2129m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Anti-Mad3 [N129/15R-rat IgG2a] - Chimeric

    Plasmid

    #231847

    Purpose

    Mammalian expression plasmid of anti-Mad3 (Human) rat IgG2a R-mab. Derived from hybridoma N129/15.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Mad3 (Homo sapiens) recombinant mouse monoclonal antibody (MXD3 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

    Plasmid

    #223143

    Purpose

    Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 111-173

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

    Plasmid

    #223144

    Purpose

    Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD1 (MBD1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 529-592

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

    Plasmid

    #223141

    Purpose

    Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1a (CBX5 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 115-177

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

    Plasmid

    #106466

    Purpose

    Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(S219A)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Changed Serine 219 to Alanine

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5 FRT TO myc Separase delta 1278-1291

    Plasmid

    #59830

    Purpose

    Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 1278-1291

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

    Plasmid

    #63565

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. AiP12927 - pAAV-AiE0078m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2927)

    Plasmid

    #230365

    Purpose

    AiE0078m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP12918 - pAAV-AiE0459m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2918)

    Plasmid

    #224016

    Purpose

    AiE0459m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP12965 - pAAV-AiE0444h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2965)

    Plasmid

    #191729

    Purpose

    AI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatum

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    mTFP1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AiP12982 - pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2982)

    Plasmid

    #191709

    Purpose

    AI plasmid ALIAS: AiP12982. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AiP12944 - pAAV-AiE0667m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2944)

    Plasmid

    #224017

    Purpose

    AiE0667m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET_Chrna6 neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid

    #101211

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AiP12946 - pAAV-AiE0697m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2946)

    Plasmid

    #230268

    Purpose

    AiE0697m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AiP12923 - pAAV-AiE0078h_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2923)

    Plasmid

    #230441

    Purpose

    AiE0078h_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AiP12926 - pAAV-AiE0078m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2926)

    Plasmid

    #230442

    Purpose

    AiE0078m_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AiP12945 - pAAV-AiE0679m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2945)

    Plasmid

    #230267

    Purpose

    AiE0679m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AiP12980 - pAAV-AiE0364h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2980)

    Plasmid

    #230272

    Purpose

    AiE0364h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AiP12939 - pAAV-AiE0496m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2939)

    Plasmid

    #230263

    Purpose

    AiE0496m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AiP12940 - pAAV-AiE0591m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2940)

    Plasmid

    #230264

    Purpose

    AiE0591m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AiP12942 - pAAV-AiE0597m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2942)

    Plasmid

    #230265

    Purpose

    AiE0597m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AiP12943 - pAAV-AiE0662m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2943)

    Plasmid

    #230266

    Purpose

    AiE0662m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AiP12947 - pAAV-AiE0454m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2947)

    Plasmid

    #230269

    Purpose

    AiE0454m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AiP12928 - pAAV-AiE0078m_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2928)

    Plasmid

    #230366

    Purpose

    AiE0078m_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AiP12925 - pAAV-AiE0078h_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2925)

    Plasmid

    #230364

    Purpose

    AiE0078h_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AiP12938 - pAAV-AiE0455m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2938)

    Plasmid

    #230141

    Purpose

    AiE0455m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AiP12941 - pAAV-AiE0370m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2941)

    Plasmid

    #230142

    Purpose

    AiE0370m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AiP12909: pAAV-AiE0445h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2909)

    Plasmid

    #230862

    Purpose

    AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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39. AiP12979 - pAAV-AiE0361h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2979)

    Plasmid

    #208120

    Purpose

    AiE0361h is an enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

    Plasmid

    #217796

    Purpose

    Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid

    #114085

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid

    #114086

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)

    Plasmid

    #114083

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.3

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pFOK

    Plasmid

    #166651

    Purpose

    Suicide vector for gene manipulation

    Depositor

    Dirk Bumann

    Article

    Cianfanelli et al BMC Microbiol. 2020 May 24;20(1):129. do

    Insert

    I-sceI, sacB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. RCAS(A) cRaxL (CC#526)

    Plasmid

    #15193

    Depositor

    Connie Cepko

    Article

    Chen et al Development. 2002 Dec . 129(23):5363-75.

    Insert

    RaxL
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

46. RCAS(B) En cRaxL deltaC (CC#523)

    Plasmid

    #15196

    Depositor

    Connie Cepko

    Article

    Chen et al Development. 2002 Dec . 129(23):5363-75.

    Insert

    RaxL
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

47. RCAS(B) En cRaxL HD (CC#520)

    Plasmid

    #15197

    Depositor

    Connie Cepko

    Article

    Chen et al Development. 2002 Dec . 129(23):5363-75.

    Insert

    RaxL
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

48. myc Rif wild-type

    Plasmid

    #38767

    Depositor

    Harry Mellor

    Article

    Pellegrin et al Curr Biol. 2005 Jan 26;15(2):129-33.

    Insert

    Rif (RHOF Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. myc Rif-QL

    Plasmid

    #38768

    Depositor

    Harry Mellor

    Article

    Pellegrin et al Curr Biol. 2005 Jan 26;15(2):129-33.

    Insert

    Rif (RHOF Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Q77L constitutively active point mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. myc Rif-TN

    Plasmid

    #38769

    Depositor

    Harry Mellor

    Article

    Pellegrin et al Curr Biol. 2005 Jan 26;15(2):129-33.

    Insert

    Rif (RHOF Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T33N dominant-negative mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge