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Showing: 1101 - 1150 of 9150 results

  6. pGAT

    Plasmid
    #112587
    Purpose
    Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence
    Depositor
    Marko Hyvönen, Johan Peränen
    Article
    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.
    Type
    Empty backbone
    Use
    Tags
    6His-GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    112587-plasmid-map-sequence-id-220166

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  8. pBad-HPII TAG206

    Plasmid
    #105846
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 206 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 206
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105846-plasmid-map-sequence-id-205551

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  9. pBad-HPII TAG568

    Plasmid
    #105845
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 568 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 568
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105845-plasmid-map-sequence-id-205540

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  10. pSB3

    Plasmid
    #82027
    Purpose
    Hybrid McpC[1-350]-Tar[267-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-Tar[267-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    82027-plasmid-map-sequence-id-175680

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  11. pSB2

    Plasmid
    #82026
    Purpose
    Hybrid McpC[1-286]-Tar[200-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-286]-Tar[200-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82026-plasmid-map-sequence-id-174389

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  12. pSB6

    Plasmid
    #82028
    Purpose
    Hybrid McpC[1-350]-YSYEH-Tar[272-553]
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-YSYEH-Tar[272-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82028-plasmid-map-sequence-id-174383

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  13. pSB10

    Plasmid
    #82039
    Purpose
    Hybrid McpS[1-295]-Tar[200-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and P. putida chemoreceptor McpS: McpS[1-295]-Tar[200-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82039-plasmid-map-sequence-id-174336

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  14. pSB7

    Plasmid
    #82029
    Purpose
    Hybrid Tar[1-43]-CitA[55-173]-Tar[184-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: Tar[1-43]-CitA[55-173]-Tar[184-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82029-plasmid-map-sequence-id-174333

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  15. pSB9

    Plasmid
    #82038
    Purpose
    Hybrid ECA0434[1-385]-Tar[267-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and P. atrosepticum chemoreceptor ECA0434: ECA0434[1-385]-Tar[267-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82038-plasmid-map-sequence-id-174332

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  19. pBAD-MBP-His-ACPs

    Plasmid
    #141140
    Purpose
    Expression of holo-[acyl-carrier-protein] synthase
    Depositor
    New England Biolabs
    Insert
    acpS
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141140-plasmid-map-sequence-id-298487

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  21. pKP579

    Plasmid
    #159598
    Purpose
    Express Cba and Cbi protein
    Depositor
    Kathleen Postle
    Insert
    cba and cbi
    Use
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    159598-plasmid-map-sequence-id-313311

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  22. pKP1912

    Plasmid
    #167600
    Purpose
    Express ExbB delta(235-244),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 235-24…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167600-plasmid-map-sequence-id-339838

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  23. pKP1940

    Plasmid
    #167599
    Purpose
    Express ExbB delta(225-234),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 225-23…
    Promoter
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    167599-plasmid-map-sequence-id-333697

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  24. pKP1965

    Plasmid
    #167598
    Purpose
    Express ExbB delta(215-224),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 215-22…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167598-plasmid-map-sequence-id-332908

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  25. pKP1964

    Plasmid
    #167597
    Purpose
    Express ExbB delta(205-214),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 205-21…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167597-plasmid-map-sequence-id-332907

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  26. pKP1963

    Plasmid
    #167596
    Purpose
    Express ExbB delta(195-204),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 195-20…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167596-plasmid-map-sequence-id-332906

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  29. pDest-566

    Plasmid
    #11517
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11517-plasmid-map-sequence-id-299808

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  34. pKP2009

    Plasmid
    #167602
    Purpose
    Express ExbB (215-224 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 215-22…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167602-plasmid-map-sequence-id-339839

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  35. pKP2010

    Plasmid
    #167603
    Purpose
    Express ExbB (235-244 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 235-24…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167603-plasmid-map-sequence-id-339840

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  36. pKP2019

    Plasmid
    #167604
    Purpose
    Express ExbB (195-204 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 195-20…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167604-plasmid-map-sequence-id-333698

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  37. pKP2040

    Plasmid
    #167650
    Purpose
    Express His6-ExbB S80A, S82A, S84A
    Depositor
    Kathleen Postle
    Insert
    his6-exbB S80A, S82A, S84A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ExbB S80A, S82A, S84A with His6 tag at N terminus
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167650-plasmid-map-sequence-id-337301

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  38. pKP2025

    Plasmid
    #167605
    Purpose
    Express ExbB (225-234 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 225-23…
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    167605-plasmid-map-sequence-id-335411

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  39. pKP2008

    Plasmid
    #167601
    Purpose
    Express ExbB (205-214 Ala),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with ala codons replacing codons 205-21…
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    167601-plasmid-map-sequence-id-335410

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  40. pNeae2

    Plasmid
    #168300
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:
    Insert
    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    Use
    Tags
    6xHis, E-tag, and myc-tag
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168300-plasmid-map-sequence-id-334450

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  42. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Article
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
    E. coli groE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    102562-plasmid-map-sequence-id-203161

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  43. pSEVA228-pro4IUPi

    Plasmid
    #122018
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pro4
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122018-plasmid-map-sequence-id-238966

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  44. pET-TRSter

    Plasmid
    #78865
    Purpose
    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
    Depositor
    Elias Arnér
    Article
    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.
    Insert
    Thioredoxin Reductase (Txnrd1 Rat)
    Use
    Tags
    Mutated E. coli-type SECIS element (3’ end of ins…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    78865-plasmid-map-sequence-id-147493

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  46. pCK301

    Plasmid
    #87767
    Purpose
    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Insert
    PrhaBAD-sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87767-plasmid-map-sequence-id-177279

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  47. pTET-IUPi

    Plasmid
    #122019
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pTET
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122019-plasmid-map-sequence-id-238972

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  48. pYihI-N-term D-N/E-Q

    Plasmid
    #233095
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to asparagine (…
    Promoter
    Available since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233095-plasmid-map-sequence-id-468389

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  49. pYihI-N-term D-A/E-L

    Plasmid
    #233097
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to alanine (A) …
    Promoter
    Available since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233097-plasmid-map-sequence-id-468390

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  50. pRAR051

    Plasmid
    #185029
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nucleotides 47-51 & 80-84 mutated rpmE 5'…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185029-plasmid-map-sequence-id-363327

    Enlarge

Showing: 1101 - 1150 of 9150 results