We narrowed to 5,876 results for: nls
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Plasmid#136901PurposeLentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)DepositorInsertFNLSHF1
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR661A,Q695A,Q926APromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-Sox2TALE-GCN4
Plasmid#120548PurposeExpress 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 geneDepositorInsertNLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-NGN2TALE-GCN4
Plasmid#120547PurposeExpress 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 eneDepositorInsertNLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FRB-TALETS3-3xFLAG-2xNLS
Plasmid#107306PurposeExpresses TALE-VEGFA-TS3 fused to FRB in mammalian cellsDepositorInsertTALE_VEGFA-TS3
UseCRISPRTags3x Flag, 2xNLS and FRBExpressionMammalianMutationPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hGCN5-NLS
Plasmid#103827PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38A-NLS
Plasmid#100792PurposeMammalian expression of Fluorescent CPI-17 neutral mutation nuclearDepositorInsertprotein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38APromoterCMVAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38E-NLS
Plasmid#100790PurposeMammalian expression of Fluorescent CPI-17 phospho mimetic nuclearDepositorInsertCPI-17-T38E-NLS (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38EPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-EGFP-NLS
Plasmid#87778PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInserteGFP
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS
Plasmid#87777PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInsertMKK7-EE
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS
Plasmid#87776PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInsertMKK4 (MAP2K4 Human)
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE NLS
Plasmid#82465PurposeExpresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminusDepositorInsertCapping Enzyme (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationdeleted amino acids Nuclear Localization SignalPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-3xFLAG-NLS-TPP1-OB
Plasmid#79060PurposeRetroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domainDepositorInsertTPP1 (TPP1 Human)
UseRetroviralTags3xFLAG and NLSExpressionMammalianMutationWild-type TPP-OB folding domainPromoterCMVAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dC-NLS
Plasmid#51840PurposeFlag-CHES1L mutant lacking the C-terminal, with remaining NLSDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSExpressionMutationcontains CHES1 amino acids 1-205PromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1LdFC-NLS
Plasmid#51838PurposeFlag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLSDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSExpressionMutationcontains CHES1 amino acids 1-109PromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseAAV and CRISPRTagsExpressionMutationPromoterEFSAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PET-HiFi SpCas9-NLS-6xHis
Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-Pfv-Sapphire-PURO
Plasmid#203654PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES-mCE NLS
Plasmid#82466PurposeExpresses cytoplasmic restricted form of myc tagged mRNA capping enzymeDepositorInsertNES mRNA capping enzyme (Rngtt HIV, Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-NLS-6xHis (gbr2101)
Plasmid#181743Purposebacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-PyronicSF/pCMV-myc-nuc
Plasmid#124814PurposeHighly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.DepositorInsertPyronicSF
UseTagsNuclear Localization SignalExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRM055 His-TEV-Cas9 6xNLS 0C
Plasmid#196246PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP Halo
Plasmid#104098PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.DepositorInsertstdMCP-Halo
UseLentiviralTagsC-terminal Halo-tag, N-terminal NLS, and Two syno…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-mScarletP2A-hLuc
Plasmid#218793PurposeAAV reporter genome plasmidDepositorInsertNLSf-mScarletP2A-hLuc
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP
Plasmid#31484DepositorInsertEF1s HA NLS Sce(opt) T2A IFP1.4
UseLentiviralTagsHA and NLSExpressionMutation"Opt" indicates the I-SceI coding seque…PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdPCP stdGFP
Plasmid#104099PurposeLentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.DepositorInsertstdPCP-stdGFP
UseLentiviralTagsN-terminal NLS, Two synonymous copies of GFP (C-t…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
UseTagsExpressionMammalianMutationD210N, catalytically inactivePromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311/NLS-Cas13d-P2A-Blast
Plasmid#212196PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40DepositorInsertCasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
UseCRISPR and LentiviralTagsHA, NLS, and blasticidin S deaminaseExpressionMammalianMutationPromoterEF-1α promoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdPCP-gfp
Plasmid#40650DepositorInserttdPCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
NLS-Laconic/pCMV-myc-nuc
Plasmid#46307Purposea nuclear-targeted, genetically-encoded sensor for lactateDepositorInsertNLS-Laconic
UseLactate biosensorTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG-VP64-SadCas9-NLS-VP64
Plasmid#135338Purpose3xFLAG 2xVP64 SadCas9 for single guide cloningDepositorInsertSadCas9
UseTags3xFLAG-VP64-SV40 NLS and NLS-VP64ExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc LbaCas12a
Plasmid#182125PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc LbaCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertfluorescent protein voltage sensor
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-LSL-nlsGFP-shRNA
Plasmid#175584PurposeConditional express shRNA with control of LoxPDepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)
Plasmid#134341PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIC3
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-NES reporter (pDN160)
Plasmid#72660PurposeNLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158DepositorInsertNLS-mCherry-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-hMCP-mScarlet-I-NLS
Plasmid#174878PurposePiggyBac transposon vector for expression of MCP-mScarlet-I-NLSDepositorInsertMCP-mScarlet-I-NLS
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-mKate2-3xNLS-IRES-Pac
Plasmid#183618PurposeMammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.DepositorInsertmKate2-3xNLS
UseTags3xNLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-YFP-NLS-STOP-L2
Plasmid#127361Purposegateway entry vector for expressing nuclear TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-6xNLS-E174R/S542R (pRG232)
Plasmid#149723PurposeLentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdMCP-gfp
Plasmid#40649DepositorInserttdMCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)
Plasmid#133801PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA4
UseTagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)
Plasmid#133802PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA5
UseTagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianMutationPromoterCMV/hUBCAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only