We narrowed to 4,209 results for: Abo;

1. Coexpressed Free deadFPPS-NES

   Plasmid

   #182492

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   deadFPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

   Plasmid

   #73440

   Purpose

   Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC-P3A2-vioD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-3xHA-SpCas9-HF1

   Plasmid

   #201952

   Purpose

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-en1FnCas9

   Plasmid

   #201947

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en1FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1369R on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-His6-FnCas9GFP

   Plasmid

   #130966

   Purpose

   expression of FnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   HA-NLS-FnCas9
   

   Use

   CRISPR

   Tags

   6xHis-mEGFP-TEV-HA-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-3xHA-FnCas9ABEmax8.17d

   Plasmid

   #201955

   Purpose

   Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304-BCL-XL-499/500R

   Plasmid

   #203575

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   silent point mutations to make resistant shRNA 49…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid

    #193721

    Purpose

    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
    

    Use

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid

    #91781

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Asp (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid

    #91733

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Ala (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFUSEss-CHIg-mM-Arg210Ala_M18

    Plasmid

    #91734

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Arg210 changed to Ala (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFUSEss-CHIg-mM-Asn209Arg_M18

    Plasmid

    #91740

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Arg (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFUSEss-CHIg-mM-Asn209Ile_M18

    Plasmid

    #91746

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ile (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Ile (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUSEss-CHIg-mM-Asn209Val_M18

    Plasmid

    #91756

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Val (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Val (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUSEss-CHIg-mM-Asn209Tyr_M18

    Plasmid

    #91755

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Tyr (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUSEss-CHIg-mM-Asn209Thr_M18

    Plasmid

    #91753

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Thr (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Thr (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFUSEss-CHIg-mM-Lys208Ala_M18

    Plasmid

    #91732

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys208Ala (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys208 changed to Ala (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFUSEss-CHIg-mM-Asn209Trp_M18

    Plasmid

    #91754

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Trp (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Trp (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge