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pScarlessHD-C-3xVHH05-DsRed

Plasmid

#171580

Purpose

For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-COX8-mScarletI

Plasmid

#129341

Purpose

Mitochondria matrix localization

Depositor

Yasushi Okada

Insert

mScarlet(i) (Addgene 85044)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3x127D01-DsRed

Plasmid

#171578

Purpose

For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2A-TORCAR

Plasmid

#175016

Purpose

TORCAR targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-TORCAR

Use

Tags

Histone H2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGAPDH::H2B-mScarlet

Plasmid

#186764

Purpose

A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

H2B-mScarlet

Use

Overexpression

Tags

H2B

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mScarlet-I

Plasmid

#104028

Purpose

Plasmid encoding 2ndVal mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mScarlet-I

Use

Low copy

Tags

Expression

Bacterial

Mutation

mScarlet-I with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCardinal

Plasmid

#197195

Purpose

Expresses the protein of mCardinal in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SUN2 mScarlet

Plasmid

#187685

Purpose

In vivo visualization of SUN2

Depositor

Mary Beckerle

Insert

SUN2 (Sun2 Mouse)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Carl-ReNL-KDEL_pcDNA3

Plasmid

#89535

Purpose

Red color variant of bright luminescent protein, targeted to the endoplasmic reticulum

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1N-mScarlet-LZ-LOV2

Plasmid

#197419

Purpose

Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 (MAPRE1 Human)

Use

Tags

A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Scarlet-Kif5a

Plasmid

#118745

Purpose

Expresses scarlet tagged Kif5a

Depositor

Beverly Koller

Insert

Kif5a cDNA (Kif5a Mouse)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Carl-YeNL-KDEL_pcDNA3

Plasmid

#89516

Purpose

Yellow color variant of bright luminescent protein, targeted to the endoplasmic reticulum

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-N-3xVHH05-DsRed

Plasmid

#171581

Purpose

For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Carl-CeNL-KDEL_pcDNA3

Plasmid

#89509

Purpose

Cyan color variant of bright luminescent protein, targeted to the endoplasmic reticulum

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHO-UAS-mScarlet-I

Plasmid

#183147

Purpose

Yeat two-hybrid fluorecent reporter for genomic integration

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

mScarlet-I

Use

Tags

Expression

Mutation

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

Plasmid

#130510

Purpose

expresses mScarlet1

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-mScarlet

Plasmid

#139503

Purpose

Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Use

Tags

mScarlet following Gly-Gly-Ser linker

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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DNA-CARzeta-GFP

Plasmid

#89344

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta (CD247, CD86 Human)

Use

Lentiviral

Tags

SNAPf tag and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pIG-747_CD19-28z_CAR_TFAP4

Plasmid

#207489

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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