We narrowed to 59,655 results for: plasmid

Showing: 441 - 460 of 59655 results

Results per page:

Sort results by:

pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Publication

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Exchangeable cassette

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

105 Articles

Enlarge
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

Plasmid

#47868

Purpose

This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a and U6

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

Plasmid

#102245

Purpose

Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

Depositor

Jacob Corn

Insert

dCas9-VPR-mCherry

Use

CRISPR and Lentiviral

Tags

NLS-VPR-mCherry

Expression

Mammalian

Promoter

CAG

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Publication

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
FH-Tet2-pEF

Plasmid

#41710

Purpose

This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

Depositor

Anjana Rao

Publication

Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

Insert

tet methylcytosine dioxygenase 2 (Tet2 Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

Enlarge
pULTRA-CNF

Plasmid

#48215

Purpose

highly efficient suppressor plasmid

Depositor

Peter Schultz

Publication

Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

Inserts

polyspecific MJ tyrosyl synthetase
tRNA

Expression

Bacterial

Mutation

Y32L, L65V, F108W, Q109M, D158G, I159A

Promoter

proK and tacI

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

Enlarge
pROSA26-PA

Plasmid

#21271

Depositor

Frank Costantini

Publication

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

Enlarge
pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pAAV.CMV.ffLuciferase.SV40

Plasmid

#105532

Purpose

AAV expression of firefly luciferase from CMV promoter

Depositor

James M. Wilson

Has service

AAV1 and AAV8

Insert

Luciferase

Use

AAV and Luciferase

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

Enlarge
pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

Enlarge
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

Enlarge
pMal-T-Avi-His/BirA

Plasmid

#102962

Purpose

Vector for in vivo biotinylation of a fusion protein in bacteria.

Depositor

Tonia Rex

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Publication

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
p212 pCMV-EGFP/RFP

Plasmid

#8389

Purpose

Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

Depositor

Jeffrey Green

Publication

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox

Expression

Mammalian

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Publication

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

Enlarge
mini-pHelper-1.0

Plasmid

#230933

Purpose

The mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

Depositor

Qizhao Wang

Publication

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Type

Empty backbone

Use

AAV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXSN16E6E7

Plasmid

#52394

Purpose

The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

Depositor

Denise Galloway

Publication

Halbert et al J Virol. 1991 Jan;65(1):473-8. doi: 10.1

Insert

HPV16 E6E7

Use

Retroviral

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

Enlarge
CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Publication

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG and linked by IRES

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

Enlarge
Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Publication

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge