We narrowed to 162,699 results for: addgene

1. pT077

   Plasmid

   #137879

   Purpose

   Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p300 KAT LIC 1M

   Plasmid

   #233587

   Purpose

   Bacterial expression of p300 KAT enzyme

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300 KAT (EP300 Human)
   

   Use

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CMV-eGFP

   Plasmid

   #193022

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tornado-RNA sensor for MALAT-1

   Plasmid

   #185406

   Purpose

   circular RNA-scaffolded sensing modules for MALAT-1

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

   Insert

   circular RNA-scaffolded sensing modules
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   U6

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV-CAG-Sun1-GFP-WPRE-pA

   Plasmid

   #220752

   Purpose

   Expression of Sun1GFP for nuclei isolation

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   Sun1-GFP-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCas9-T2A-GFP

   Plasmid

   #78548

   Purpose

   Cas9 protein with GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Parkin

   Plasmid

   #213547

   Purpose

   Transient mammalian expresssion of untagged Parkin

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-kdrl:mCherry-caax

   Plasmid

   #194287

   Purpose

   Expression plasmid for zebrafish or Danionella cerebrum

   Depositor

   Pui-Ying Lam

   Article

   Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

   Insert

   kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
   

   Use

   Zebrafish or danionella cerebrum expression

   Tags

   mCherryCAAX from the Tol2kit (Kwan et al., 2007)

   Expression

   Mutation

   Promoter

   kdrl(flk1)

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-XMAS-1xStop

    Plasmid

    #164411

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TNFa-GFP

    Plasmid

    #163660

    Purpose

    Expresses GFP tagged TNFa in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    tumor necrosis factor (TNF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158972

    Purpose

    Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE puro human IRS-1

    Plasmid

    #11025

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. XRE-H2B-eGFP

    Plasmid

    #182294

    Purpose

    Fluorescent reporter for AhR activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    Xenobiotic Response Element (CYP1A1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    XRE

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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