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pDRAV-1

Plasmid

#26746

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Type

Empty backbone

Use

Cre/Lox; Flp/frt; phic31/attb-attp

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pCM5.44

Plasmid

#19389

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

cep-1 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pAC1822_pCR8-dCasRx

Plasmid

#118656

Purpose

Gateway Donor; dCasRx ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBTM116-D9

Plasmid

#111232

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Article

Worseck et al Methods Mol Biol. 2012;812:63-87. doi: 1

Insert

ccdB-cassette

Expression

Yeast

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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p{3P3-dsRed::FRT-RC,attB}

Plasmid

#182177

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::FRT-RC,attB}

Plasmid

#182175

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-mTurquoise::FRT-RC,attB}

Plasmid

#182181

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCM5.57

Plasmid

#19378

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

spe-11 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pSCM167-Flag-hSFRP1(aa181-314)

Plasmid

#189551

Purpose

Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.

Depositor

Haian Fu

Insert

truncated secreted frizzled related protein 1 (SFRP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

contains aa181-314

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDRAV-3

Plasmid

#26748

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Type

Empty backbone

Use

Cre/Lox; Flp/frt; phic31/attb-attp

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pME-6MTnlstdTomato2A

Plasmid

#67685

Purpose

Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

tdTomato

Tags

2A and 6xMyc-NLS

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_763_4xUAS_UPS_hkCP

Plasmid

#71171

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

763_4xUAS_UPS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-Site2

Plasmid

#24513

Depositor

Kazuo Kinoshita

Article

Uemura et al PLoS One. 2010 . 5(3):e9846.

Insert

Split RFP-GFP chimera

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-Site6

Plasmid

#24515

Depositor

Kazuo Kinoshita

Article

Uemura et al PLoS One. 2010 . 5(3):e9846.

Insert

Split RFP-GFP chimera

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 26, 2010

Availability

Academic Institutions and Nonprofits only

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pDRAV-4

Plasmid

#26749

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Type

Empty backbone

Use

Cre/Lox; Flp/frt; phic31/attb-attp

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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huβB1cry:mAppleCAAX

Plasmid

#122451

Purpose

Human BB1 crystallin promoter driving mApple-CAAX in Tol2 destination vector

Depositor

Thomas Schilling

Article

Vorontsova et al J Vis Exp. 2019 May 6;(147). doi: 10.379

Insert

Human BB1 crystallin:mApple_CAAX

Expression

Bacterial

Promoter

300bp Human Beta B1 crystallin promoter

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLEELA

Plasmid

#79752

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Marc Jakoby

Article

Jakoby et al FEBS Lett. 2004 Nov 19;577(3):528-34.

Type

Empty backbone

Expression

Plant

Promoter

2xPro35S

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pQHD

Plasmid

#48102

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

HA tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSGE_764_4xUAS_DOS_hkCP

Plasmid

#71172

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

764_4xUAS_DOS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pQFD

Plasmid

#48101

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-Site5

Plasmid

#24514

Depositor

Kazuo Kinoshita

Article

Uemura et al PLoS One. 2010 . 5(3):e9846.

Insert

Split GFP-RFP chimera

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG371

Plasmid

#128410

Purpose

Cuminic acid-inducible system

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pspank(C)

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_HuB

Plasmid

#65764

Purpose

expresses RFP-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pLG231

Plasmid

#124369

Purpose

IPTG inducible system (gfpmut2 with Ppsank and riboJ) for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2

Expression

Bacterial

Promoter

Pspank

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSGE_91_4xUAS_dCP

Plasmid

#71169

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

91_4xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM5.51

Plasmid

#19382

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

rme-2 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pEV9.03

Plasmid

#37495

Depositor

Geraldine Seydoux

Article

Voronina et al Development. 2010 May;137(9):1441-50. Ep

Insert

npp-16 (npp-16 Nematode)

Use

RNAi

Expression

Worm

Mutation

nts 162-673 of cds

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pQYD

Plasmid

#48104

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

SYFP2

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC180

Plasmid

#19616

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC267

Plasmid

#19618

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)-iA-FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM5.60

Plasmid

#19374

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fog-1 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
FoRC.PGa

Plasmid

#84584

Purpose

Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Expression

Insect

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FoRC.PRa

Plasmid

#84585

Purpose

Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Expression

Insect

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM5.41

Plasmid

#19392

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

lip-1 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM5.66

Plasmid

#19370

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pos-1 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_539_2xUAS_dCP

Plasmid

#71168

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

539_2xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM5.65

Plasmid

#19371

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mes-3 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR3.06

Plasmid

#19368

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

daz-1 ORF+3' in pDONR201

Expression

Worm

Available Since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR3.01

Plasmid

#19369

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

spn-4 ORF+3' in pDONR201

Expression

Worm

Available Since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM5.42

Plasmid

#19390

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fog-2 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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