We narrowed to 12,157 results for: pcDNA
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Plasmid#59388PurposeDestination vector with RBPMS for stable cell line generationDepositorAvailable SinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only
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Opto-GPR19
Plasmid#106007PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)DepositorInsertOpto-GPR19 (GPR19 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3 delta Exon2
Plasmid#100782PurposeMammalian expression of BNIP3 delta Exon2 splice variantDepositorInserthuman-BNIP3 delta Exon2 (BNIP3 Human)
TagsHAExpressionMammalianMutationExon2 deletionPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFP-PCRD-FRB
Plasmid#87449Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channelsDepositorInsertPCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
Tags6xHis-T7, CFP, and FRBExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRD439
Plasmid#168465PurposeFor the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mTurquoise2-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-1058
Plasmid#135347PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminusDepositorInsertnSpyCas9-hAIDx-ID-1058-UGI
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR75
Plasmid#106032PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)DepositorInsertOpto-GPR75 (GPR75 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEMAX-NLS-P2A_BLAST
Plasmid#135355PurposeExpression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertABEMax-2XNLS
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRT2-H2B-EGFP-V5-2A (IG157)
Plasmid#99623PurposeTo clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassetteDepositorInsertH2B-EGFP-V5
TagsT2AExpressionMammalianAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR61
Plasmid#106027PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)DepositorInsertOpto-GPR61 (GPR61 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-EOMESwt-IDR
Plasmid#145231PurposeExpresses fusion of GAL4 DNA-binding domain and EOMESwt IDRDepositorInsertEOMESwt-IDR (EOMES Human)
ExpressionMammalianAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN1
Plasmid#92243PurposeTALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2DepositorInsertTALEN
UseTALENPromoterCMVAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN2
Plasmid#92244PurposeTALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1DepositorInsertTALEN
UseTALENPromoterCMVAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-BHLHE41wt-IDR
Plasmid#145229PurposeExpresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDRDepositorInsertBHLHE41wt-IDR (BHLHE41 Human)
ExpressionMammalianAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13wt-IDR
Plasmid#145240PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13wt IDRDepositorInsertHOXA13wt-IDR (HOXA13 Human)
ExpressionMammalianAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE3320
Plasmid#107529PurposeExpresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.DepositorInserthLbCpf1(RVR)
Tags3xHA and NLSExpressionMammalianMutationG532R, K538V, Y542RPromoterCMVAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR88
Plasmid#106039PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR88; Opto-GPR88; D5)DepositorInsertOpto-GPR88 (GPR88 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR119
Plasmid#106040PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR119; Opto-GPR119; D6)DepositorInsertOpto-GPR119 (GPR119 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL649 CHYRON2 integration construct
Plasmid#126446PurposeTo integrate the CHYRON2 locus at AAVS1 in human cells.DepositorInsertspU6-CHYRON2 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-mVenus-TEV
Plasmid#105774PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmVenus-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR135
Plasmid#106043PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR135; Opto-GPR135; D9)DepositorInsertOpto-GPR135 (GPR135 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRT3-Mb2-HA-mTfp1-2A (IG155)
Plasmid#99621PurposeTo clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2ADepositorInsertMb2-HA-mTfp1
ExpressionMammalianAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT1-Mb2YFP-2A (IG153)
Plasmid#99619PurposeTo clone gene of interest downstream of FRT1-Mb2YFP-2A cassetteDepositorInsertMb2EYFP
TagsT2AExpressionMammalianAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT2-Mb2Tomato-2A (IG154)
Plasmid#99620PurposeTo clone gene of interest downstream of FRT2-Mb2Tomato-2A cassetteDepositorInsertMb2Tomato
TagsT2AExpressionMammalianAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT3-HA-H2B-Cerulean-2A (IG158))
Plasmid#99624PurposeTo clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassetteDepositorInsertHA-H2B-Cerulean
TagsT2AExpressionMammalianAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-FFluc-GPD1L-3'UTR WT
Plasmid#32488DepositorAvailable SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-FFluc-GPD1L-3'UTR Seed Mut
Plasmid#32490DepositorAvailable SinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
AcrVA1_AsLOV2-S103ΔELS
Plasmid#246049PurposeExpression of AcrVA1-AsLOV2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables optogenetic control of MbCas12aDepositorInsertAcrVA1_AsLOV2-S103ΔELS
UseCRISPR and Synthetic BiologyExpressionMammalianMutationΔELSAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
AcrVA1_cpGR2(GGS5)-S103ΔELS
Plasmid#246050PurposeExpression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12aDepositorInsertAcrVA1_cpGR2-S103ΔELS
UseCRISPR and Synthetic BiologyExpressionMammalianMutationΔELSAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66
Plasmid#246052PurposeExpression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9DepositorInsertAcrIIA4_cpGR2-ΔN64/Q65/E66
UseCRISPR and Synthetic BiologyExpressionMammalianMutationΔN64/Q65/E66Available SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1-H2B-mRFP1-pcdk1-EGFP1-FRT
Plasmid#247334PurposeExpresses histone H2B fused with mRFP1, together with monomeric EGFPDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-Src Y527E
Plasmid#246428PurposeExpresses Src mutant Y527E (aka Y530E) in Mammalian cellsDepositorAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHCFnc/V5
Plasmid#247003PurposeExpresses non cleavable HCF-1 with carboxy terminal V5 tag under control of the CMV promoterDepositorInsertHCF-1 (HCFC1 Human)
TagsV5 tagExpressionMammalianMutationGlu to Ala change at amino acid positions 1019,10…Available SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag- Src H492A
Plasmid#246425PurposeExpresses Src mutant H492A (aka H495A) in Mammalian cellsDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-Src Y527A
Plasmid#246429PurposeExpresses Src mutant Y527A (aka Y530A) in Mammalian cellsDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-Src H201A
Plasmid#246424PurposeExpresses Src mutant H201A (aka H204A) in Mammalian cellsDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-Src E524A
Plasmid#246426PurposeExpresses Src mutant E524A (aka E527A) in Mammalian cellsDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-Src C185A
Plasmid#246423PurposeExpresses Src mutant C185A or C188A in Mammalian cellsDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAD120-CHIP-G132N-myc-His
Plasmid#236053PurposeMammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tagDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAD000-CHIP-K30A-myc-His
Plasmid#236052PurposeMammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tagDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CHGA-eGFP
Plasmid#228569PurposeExpresses GFP-tagged human Chromogranin A in mammalian cellsDepositorAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-T453D
Plasmid#235209PurposeExpresses GRP78 with T453D mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
TagsFLAGExpressionMammalianMutationChange Threonine 453 to Aspartic AcidAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-R197H
Plasmid#235208PurposeExpresses GRP78 with R197H mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
TagsFLAGExpressionMammalianMutationChange Arginine 197 to HistidineAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-G227D
Plasmid#235207PurposeExpresses GRP78 with G227D mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
TagsFLAGExpressionMammalianMutationChange Glycine 227 to Aspartic AcidAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-NLS-Mut
Plasmid#235206PurposeExpresses GRP78 with mutated NLS in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
TagsFLAGExpressionMammalianMutationMutate the NLS sequenceAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pAK208_2xBRD9BD-IFP2.0C
Plasmid#233734PurposeExpresses BRD9 Bromodomain as tandem double domain fused to C-terminal part of IFP2.0 for histone acetylation detection in BiAD sensorsDepositorInsertBRD9 (BRD9 Human)
TagsIFP2.0 C-terminal partExpressionMammalianMutationBromodomain cloned twice in tandemPromoterCMVAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK208_2xBRD9BD_Y57A-IFP2.0C
Plasmid#233733PurposeExpresses BRD9 Bromodomain as tandem double domain Y57A mutant fused to C-terminal part of IFP2.0 as negative control for plasmid 233734DepositorInsertBRD9 (BRD9 Human)
TagsIFP2.0 C-terminal partExpressionMammalianMutationBromodomain cloned twice in tandem with Y57A muta…PromoterCMVAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only