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mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Tags

6xHis and mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pDisplay FLIPE-1m

Plasmid

#13548

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159857

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Plxna2-Fc-His

Plasmid

#72123

Purpose

Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna2 (Plxna2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-HADH2

Plasmid

#67863

Purpose

bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

HADH2 (HSD17B10 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Tom20-mChF-AIP(TA)

Plasmid

#61513

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutation

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP with TA mutation (see comments), Tom20, and m…

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
human CD40 promoter

Plasmid

#53433

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Nguyen et al J Biol Chem. 2002 Apr 19;277(16):13796-8

Insert

CD40 promoter (CD40 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CD40

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA1ΔC

Plasmid

#30306

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 ΔC (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids Pro 526 to Gln 548

Available Since

Aug. 26, 2011

Availability

Academic Institutions and Nonprofits only
pSVE Grb2 myc 49L/203R

Plasmid

#26087

Depositor

Bob Weinberg

Article

Egan et al Nature. 1993 May 6. 363(6424):45-51.

Insert

Grb2 49L/203R (GRB2 Human)

Tags

myc

Expression

Mammalian

Mutation

49L/203R

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
AiP12045-pAAV-3xSP10ins-AiE0359h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2045)

Plasmid

#163504

Purpose

AiE0359h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ cortical interneurons and cerebellar Purkinje cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

MinRho

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134900

Plasmid

#151911

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

d1-15 d1209-1273 R682A R685G K986P V987P

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST40-2XHA-Src-K298M

Plasmid

#140314

Purpose

Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT

Plasmid

#112705

Purpose

Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/Venus-mDFCP1

Plasmid

#58742

Purpose

Expresses Venus-mDFCP1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

Venus

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pEGFPm-DAD

Plasmid

#25410

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

mDia2 Amino Acids 1031-1171; Q1091P mutation

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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p6604 MSCV-IP N-HAonly PP2CA

Plasmid

#35005

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PP2CA (PP2CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pUC57-codon-modified HTK

Plasmid

#34563

Depositor

David Anderson

Article

Lo et al Neuron. 2011 Dec 22;72(6):938-50.

Insert

codon-modified HTK

Expression

Bacterial

Mutation

codon modified HTK using mouse codon usage table

Promoter

pUC57 bacteria promoter

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

Plasmid

#173030

Purpose

To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

Depositor

Mohamed Fareh

Article

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

SARS-CoV-2 Nucleocapsid phosphoprotein

Use

Lentiviral

Tags

3xHA and IRES-mcherry

Expression

Mammalian

Promoter

Gag (truncated)

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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