We narrowed to 34,221 results for: CaS

1. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

   Plasmid

   #234724

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   T2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

   Plasmid

   #233413

   Purpose

   LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   LIDARSensor(mCherry->Casp9-2A-GFP)-hygro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

   Plasmid

   #233033

   Purpose

   To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

   Depositor

   Jonathan Ploski

   Insert

   DjCas13d and mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

   Plasmid

   #233031

   Purpose

   To express a DjCas13d-compatible gRNA

   Depositor

   Jonathan Ploski

   Insert

   DjCas13d-compatible gRNA expression cassette
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

   Plasmid

   #233038

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter

   Depositor

   Jonathan Ploski

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

   Plasmid

   #233034

   Purpose

   To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

   Depositor

   Jonathan Ploski

   Insert

   hfCas13d and mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

   Plasmid

   #233032

   Purpose

   To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

   Depositor

   Jonathan Ploski

   Insert

   CasRx and mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

   Plasmid

   #221448

   Purpose

   Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3

   Depositor

   Omar Akbari

   Inserts

   wtCas9-NG
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

   Plasmid

   #227009

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

    Plasmid

    #227010

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

    Plasmid

    #227011

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

    Plasmid

    #227012

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 783-Rx-mU6: RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228361

    Purpose

    mU6-driven expression of RfxCas13d gRNAs and arrays. Contains AarI sites for guide cloning.

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

    Plasmid

    #227773

    Purpose

    Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and tp1

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

    Plasmid

    #227774

    Purpose

    Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Insert

    loxP-dsR-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Anti-CASPR/Neurexin IV [K65/35R-rat IgG2a]

    Plasmid

    #222178

    Purpose

    Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

    Plasmid

    #223146

    Purpose

    Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    NIPP1 (PPP1R8 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 143-224

    Promoter

    EF1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

    Plasmid

    #223145

    Purpose

    Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD2 (MBD2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 176-231

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

    Plasmid

    #223143

    Purpose

    Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 111-173

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

    Plasmid

    #223144

    Purpose

    Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD1 (MBD1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 529-592

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge